Biochemistry Exam 1 Flashcards
Value:
35-55 g/L
OR
3.5-5.5 g/dL
Analyte:
Albumin
Analyte:
Albumin
Value:
35-55 g/L
OR
3.5-5.5 g/dL
Value:
6.0-7.8 g/dL
Analyte:
Protein Total
Analyte:
Protein Total
Value:
6.0-7.8 g/dL
Value:
2.5-3.5 g/dL
Analyte:
Globulins
Analyte:
Globulins
Value:
2.5-3.5 g/dL
Value:
640-1430 mg/dL
Analyte:
IgG
Analyte:
IgG
Value:
640-1430 mg/dL
Value:
70-300 mg/dL
Analyte:
IgA
Analyte:
IgA
Value:
70-300 mg/dL
Value:
20-140 mg/dL
Analyte:
IgM
Analyte:
IgM
Value:
20-140 mg/dL
Value:
< 8 mg/dL
Analyte:
IgD
Analyte:
IgD
Value:
< 8 mg/dL
Value:
0.01-0.04 mg/dL
Analyte:
IgE
Analyte:
IgE
Value:
0.01-0.04 mg/dL
Value:
8-20 mg/dL
Analyte:
Blood Urea Nitrogen
(BUN)
Analyte:
Blood Urea Nitrogen
(BUN)
Value:
8-20 mg/dL
Value:
0.7-1.3 mg/dL
Analyte:
Creatinine
Analyte:
Creatinine
Value:
0.7-1.3 mg/dL
Value:
2.5-8 mg/dL
Analyte:
Uric Acid
Analyte:
Uric Acid
Value:
2.5-8 mg/dL
Value:
36-92 U/L
Analyte:
Alkaline phosphatase
Analyte:
Alkaline phosphatase
Value:
36-92 U/L
Value:
0-35 U/L
Analyte:
Alanine Aminotransferase
(ALT)
Analyte:
Alanine Aminotransferase
(ALT)
Value:
0-35 U/L
Value:
0-35 U/L
(NOT ALT)
Analyte:
Aspartate Aminotransferase
(AST)
Analyte:
Aspartate Aminotransferase
(AST)
0-35 U/L
Value:
0-130 U/L
Analyte:
Amylase
Analyte:
Amylase
Value:
0-130 U/L
Value:
60-100 U/L
Analyte:
Lactate dehydrogenase
(LD orLDH)
Analyte:
Lactate dehydrogenase
(LD or LDH)
Value:
60-100 U/L
Value:
0-30 U/L
Analyte:
Gamma Glutamyltransferase
(GGT)
Analyte:
Gamma Glutamyltranferase
(GGT)
Value:
0-30 U/L
Value:
70-100 mg/dL
Analyte:
Glucose
Analyte:
Glucose
Value:
70-100 mg/dL
Value:
< 200 mg/dL
Analyte:
Cholesterol - total
Analyte:
Cholesterol - total
Value:
< 200 mg/dL
Value:
< 130 mg/dL
Analyte:
Low-density lipoprotein
(LDL)
Analyte:
Low-density lipoprotein
(LDL)
Value:
< 130 mg/dL
Value:
> 40 mg/dL
Analyte:
High-density lipoprotein
(HDL)
Analyte:
High-denisty lipoprotein
Value:
> 40 mg/dL
Value:
< 150 mg/dL
Analyte:
Triglyceride
Analyte:
Triglyceride
Value:
< 150 mg/dL
Value:
8.6-10.0 mg/dL
Analyte:
Calcium, Total
Analyte:
Calcium, Total
Value:
8.6-10.0 mg/dL
Value:
4.1-4.9 mg/dL (plasma)
Analyte:
Calcium, Ionized
Analyte:
Calcium, Ionized
Value:
4.1-4.9 mg/dL (plasma)
Value:
98-107 mmol/L
Analyte:
Chloride
Analyte:
Chloride
Value:
98-107 mmol/L
Value:
2.4-4.4 mg/dL
Analyte:
Phosphorus
Analyte:
Phosphorus
Value:
2.4-4.4 mg/dL
Value:
135-145 mmol/L
Analyte:
Sodium
Analyte:
Sodium
Value:
135-145 mmol/L
Value:
10-20 mmol/L
Analyte:
Anion Gap
Analyte:
Anion Gap
Value:
10-20 mmol/L
Value:
275-292 mOsm/kg
Analyte:
Osmolality
Analyte:
Osmolality
Value:
275-295 mOsm/kg
Value:
5-10 mOsm/kg
Analyte:
Osmolal Gap
Analyte:
Osmolal Gap
Value:
5-10 mOsm/kg
Value:
3.5-5.1 mmol/L
Analyte:
Potassium
Analyte:
Potassium
Value:
3.5-5.1 mmol/L
Value:
0.63-1.0 mmol/L
Analyte:
Magnesium
Analyte:
Magnesium
Value:
0.63-1.0 mmol/L
Value:
22-29 mmol/L
Analyte:
Bicarbonate
(HCO3-)
Analyte:
Bicarbonate
(HCO3-)
Value:
22-29 mmol/L
Value:
7.35-7.45 mm Hg
Analyte:
pH
(arterial blood gas)
Analyte:
pH
(arterial blood gas)
Value:
7.35-7.45 mm Hg
Value:
35-45 mm Hg
Analyte:
PCO2
(arterial blood gas)
Analyte:
PCO2
(arterial blood gas)
Value:
35-45 mm Hg
Value:
85-105 mm Hg
Analyte:
PO2
(arterial blood gas)
Analyte:
PO2
(arterial blood gas)
Value:
85-105 mm Hg
Value:
23-29 mmol/L
Analyte:
CO2
(venous)
Analyte:
CO2
(venous)
Value:
23-29 mmol/L
Value:
< 5 ug/dL
Analyte:
Lead
Analyte:
Lead
Value:
< 5 ug/dL
Value:
15-200 ng/mL
Analyte:
Ferritin
Analyte:
Ferritin
Value:
15-200 ng/mL
Value:
60-160 ug/dL
Analyte:
Iron
Analyte:
Iron
Value:
60-160 ug/dL
Value:
20%-50%
Analyte:
Transferrin Saturation %
Analyte:
Transferrin Saturation %
Value:
20%-50%
Value:
0.3-1.2 mg/dL
Analyte:
Bilirubin - Total
Analyte:
Bilirubin - Total
Value:
0.3-1.2 mg/dL
Value:
0-0.3 mg/dL
Analyte:
Bilirubin - Direct
Analyte:
Bilirubin - Direct
Value:
0-0.3 mg/dL
Name the Equation

Henderson-Hasselbalch Equation
This equation mathematically describes the dissociation characteristics of weak acids and bases
Henderson-Hasselbalch equation

The following equation gives what result?

Anion gap
What equation is calculated by the concentration difference between commonly measured cations (Na+K) and commonly measured anions (Cl+HCO3)?
Anion gap

What is the following equation used to calculate?

Osmolality
What is a physical property of a solution that is based on the concentration of solutes (expressed as millimoles) per kilogram of solvent (w/w)?
Osmolality
The following equation will give you what result?

Transferrin Saturation
What is the equation for creatinine clearance?

How do you calculate the percent coefficient of variation (%CV)?

How do you calculate standard deviation?

What is this equation for?

Calculating standard deviation
What is this equation used for?

Calculating the percent coefficient variation
How do you calculate total cholesterol?

The following equation will give you what result?

Total cholesterol
How do you calculate VLDL?

The following equation gives you which analyte result?

VLDL
How do you calculate LDL when given total cholestrol, HDL, and Triglyceride results?

The following equation will give you what analyte result?

LDL
Low-density lipoprotein
What is another name for the following equation?

Friedewald formula
What is the Friedewald formula?

How do you calculate Absorbance when given %T?
2 - log10(%T)

What is Beer’s Law?
A = abc
A = Absorbance a = molar absorptivity in L/[(mole)(cm)] b = path length in cm c = concentration of the analyte (sample) in mol/L
What is a class A fire?
Ordinary combustible solid materials (paper, wood, etc.)
What is a class B fire?
Flammable liquids/gases and petroleum products
What is a class C fire?
Energized electrical equipment
What is a class D fire?
Combustible/reactive metals (magnesium, sodium, etc.)
What type of extinguishers are used on class A fires?
- Pressurized water
- Foam
- Multipurpose dry chemical
What type of extinguishers are used on Class B and Class C fires?
- Multipurpose dry chemical
- Carbon dioxide
- Halogenated hydrocarbon (for computers)
What type of extinguishers are used on Class D fires?
- Dry chemical extinguishers (trained firefighters only)
Which of the following standards require that MSDSs are accessible to all employees who come in contact with a hazardous compound?
A. Bloodborne Pathogen Standard
B. Personal Protection Equipment Standard
C. Hazardous Communication Standard
D. CDC Regulations
C. Hazardous Communication Standard
Chemicals should be stored:
A. Inside a safety cabinet with proper ventilation
B. According to their chemical properties and classification
C. Inside a fume hood, if toxic vapors can be released when opened
D. Alphabetically, for easy accessibility
B. According to their chemical properties and classification
Proper personal protective equipment (PPE) in the chemistry laboratory for routine testing includes:
A. Safety glasses for individuals not wearing contact lenses
B. Gloves with rubberized sleeves
C. Impermeable lab coat with eye/face protection and appropriate disposable gloves
D. Respirators with HEPA filter
C. Impermeable lab coat with eye/face protection and appropriate disposable gloves
A fire caused by a flammable liquid should be extinguised using which type of extinguisher?
A. Halogen
B. Class B
C. Class C
D. Pressurized water
B. Class B
Which of the following is the proper means of disposal for the type of waste?
A. Microbiologive waste by steam sterilization
B. Radioactive waster by incineration
C. Xylene into the sewer system
D. Mercury by burial
A. Microbiologive waste by steam sterilization
What are the major contributing factors to repetitive strain injuries?
A. Position/posture, applied force, and frequency of repitition
B. Temperature and vibration
C. Fatigue, clumsiness, and lack of coordination
D. Inattention on the part of the laboratorian
A. Position/posture, applied force, and frequency of repitition
Which of the following are examples of nonionizing radiation?
A. Gamma rays and x-rays
B. Alpha and beta radiation
C. Ultraviolet light and microwaves
D. Neutron radiation
C. Ultraviolet light and microwaves
One liter of 4 N sodium hydroxide (strong base) in a glass 1 L beaker accidentally fell and spilled on the laboratory floor. The first step is to:
A. Squirt water on the spill to dilute the chemical
B. Throw some kitty litter on the spill
C. Alert and evacuate those in the immediate area out of harms way
D. Call 911
E. Neutralize with absorbing materials in a nearby spill kit
C. Alert and evacuate those in the immediate area out of harms way
Of the following, which is NOT reportable to the Department of Labor?
A. A laboratorian with a persistent cough that is only triggered at work
B. A laboratorian that tripped in the lab and hit her head on the lab bench rendering her unconscious
C. A laboratorian that experienced a chemical burn
D. A laboratorian that was stuck by a contaminated needle after performing phlebotomy on a patient
E. A laboratorian that forgot to wear his lab coat and gloves while diluting patient serum
A. A laboratorian with a persistent cough that is only triggered at work