Metabolic Pathways Flashcards
Where do you synthesize? (Anabolic)
Cytoplasm
Where do you break something down? (Catabolism)
Mitochondria
What is the substrate that starts and the product for Heme synthesis?
Succinyl -CoA is the substrate that starts (allosteric activator)
Ferrochelatase is the product which adds iron in the heme ring.
What amino acid and vitamin do you need for heme synthesis?
Glycine and Vit B6
If you are missing the mannose 6 phosphate label then no enzymes will end up in the lysosomes .. what DZ am I ?
I cell
Which complexes use heme ?
3 and 4
Statins destroy what?
Coenzyme Q10 aka ubiquinone
Copper is apart of which complex ?
complex IV aka cytochrome oxidase complex
Which complex phosphorlyates ?
Complex V
what blocks complex II ?
malonate
How do uncouplers cause Malignant hyperthermia and neuroleptic malignant syndrome?
allows ETC to continue but ATP synthesis stops and leaves muscle contracted
Which nucleotides have the tightest bond? loosest?
AT have the loosest bonds (double) and the most rapidly dividing cells
GC has the tightest (three) bonds and is found in the brain.
To isolate DNA use?
thymidine - southern blot
to isolate RNA use?
uracil - northern blot
What is the substrate that starts and the product for Purine synthesis?
Start with Ribose 5 phosphate from the Pentose Pathway
PRPP is high when?
de novo, higher Km
PRPP is low when?
Salvage, lower Km
When is de novo preferred?
periods of rapid growth
CA
0-2, 4-7, puberty, pregnancy
rRNA are synthesized where?
nucleolus
they are most abundant
mRNA
synthesized during transcription
DNA –> mRNA
3’ - poly A tail
5’ - Guanosine cap
SNRPS - small nuclear ribonuclear proteins
smallest
responsible for splicing of mRNA during posttranscriptional modification
tRNA is used in?
translation
the anticodon is responsible for finding AA
3’ contains CCA and cost 2 GTPs
RNA polymerase 1 synthesizes
rRNA
RNA polymerase 2 synthesizes
mRNA
