Media Sterilization

Question 1

Common sterilization methods

for animal/insect cell culture media and microbial media and why.

Answer 1

Filtration: Commonly used with animal cell culture medium where heat would result in severe component degradation
Heat: Cheaper, Usually used for microbial medium sterilization

Question 2

Commonly used types of batch and continous heat sterilization equipment

Answer 2

Use at high temperature for short time for less nutrient degradation.