Media Formulation Flashcards
Defined (Synthetic) and Complex (Natural) Media
Defined: Exact composition of medium is known, prepared from pure components
Complex Contains ingredient that are not completely defined chemically
List and Discuss Complex Carbon and Nitrogen Sources
Media formulation
Carbon Starch, Whey, Molasses
Nitrogen Pharmamedia, Corn Steep Liquor, Soybean Meal, Yeast Extract,
Optimal medium formulation for microbial cell growth vs optimal medium formulation for product formation.
Are they the same?
May Differ Significantly
Most product is formed during stationary phase of cell growth.
Major Issues with formualting animal and insect cell culture media
Including serum-containing and serum-free media
Serum Contains: Lipid, growth factors, proteins, protective factors.
Serum Free: Replace components of Serum with the following.
Insect Cell: Lipids and protective agent
Mammalian cell: Lipids, protective agent, and growth factors
