Mastitis Part A GC

Question 1

Answer 1

Streptococcus agalactiae on blood agar with S.aureus beta-hemolysis streaked down center . CAMP reaction is clearing in area of hemolysis streak

Question 2

Answer 2

Beta-hemolytic Streptococcus agalactie on blood agar containing esculin

Question 3

Answer 3

CAMP positive S. Iberia on blood agar with esculent , streaked with S.aureus beta-helolysin

Question 4

Answer 4

Staphylococcus aureus producing zones of both complete and incomplete hemolysis on blood agar

Question 5

Answer 5

Staphylococcus spp on blood agar . No hemolysis

Question 6

Answer 6

E.coli on blood sugar

Question 7

Answer 7

E.coli on MacConkey agar. Pink colony pigment is due to lactose fermentation. Note the precipitation of bile salts.

Question 8

Answer 8

Klebsiella spp on blood agar.

Question 9

Answer 9

Klebsiella spp on MacConkey agar. Wet, mucoid colonies are due to capsule .

Question 10

Answer 10

Serratia spp. on split plate with blood agar on the left and MacConkey agar on the right . The red color is due to colony pigment, not lactose fermentation.

Question 11

Answer 11

Pseudomonas spp. on blood agar streaked with S.aureus beta-hemolysin

Question 12

Answer 12

Proteus spp on blood agar showing swarming or spreading over surface of agar

Question 13

Answer 13

Pausterella spp on blood agar showing lobate edges on most colonies.

Question 14

Summary table Gram Negative growth

Answer 14

Question 15

Answer 15

Mycoplasma spp colonies with typical fried egg appearance

Question 16

Answer 16

Methylene blue stained smear of yeast (small ) and Prototheca spp ( large )

Question 17

Answer 17

Prototheca spp culture at 48 hours on split plates. Blood agar on the left and Sabouraud Dextrose Agar on the right, showing dry, granular surface of colony.

Question 18

Answer 18

Corynebacterium bovis on blood agar

Question 19

Do you remember CMT threshold for different scores ?

Answer 19

Question 20

Do you remember Somatic Cell Score?

Answer 20

Question 21

Milk yield & SCS?

Answer 21

Question 22

SCC & milk loss?

Answer 22

Question 23

Differentiation of streptococci ?

Answer 23

Question 24

What is the definition of bimodal milking ?

Answer 24

Bimodal milk flow periods are defined as those when milk flow is interrupted after removal of cisternal milk but before alveolar milk reaches the gland cistern (Bruckmaier and Blum, 1998)

Bimodal milk flow, characterised by a period of reduction or even transient absence of milk flow in the beginning of milking (Fernandes et al. 2023- Animal)

Question 25

Do you remember interpretation bulk tank milk ?

Answer 25