Mass Spectrometry in Proteomics and Pathogen Identification

Question 1

uses the known sequences of the entire human genome for determining the role of genetics in certain human diseases

Answer 1

Genomics

Question 2

the investigation of the protein products encoded by these genes

Answer 2

Proteomics

Question 3

often used in the discovery of new biochemical markers

Answer 3

“shotgun” approach

Question 4

can be trypsin digested and separated by HPLC or techniques such as two dimensional electrophoresis can be used to separate proteins into individual spots or bands

Answer 4

Proteins

Question 5

used for the analysis of biomolecules, such as peptides and proteins.

Answer 5

MALDI-TOF MS

Question 6

The solvent is ______ and the plate is introduced into the vacuum system of the MALDI-TOF analyze

Answer 6

dried

Question 7

Ions from the sample are focused into the

Answer 7

mass spectrometer

Question 8

The molecular weight of the proteins acquired by mass spectrum is used to determine the identity of the sample and is helpful in determining posttranslational modifications that may have occurred.

Answer 8

MALDI-TOF AND SELDI-TOF MS

Question 9

A modification of MALDI-TOF MS is SELDI-TOF MS, in which proteins are directly captured on a _____________ _______ without the need of sample preparation

Answer 9

chromatographic biochip

Question 10

MS has primarily been utilized in __________ and ____________ sections for the analysis of small molecules and proteins, high-resolution MS can be used for the identification of pathogens in modern ____________ laboratories.

Answer 10

chemistry; toxicology; microbiology

Question 11

This protein “____________” is composed of mainly ribosomal proteins and can be compared with a digital database of species

Answer 11

fingerprint

Question 12

Because MALDI-TOF MS requires inexpensive reagents and only takes _________ minutes per run, it is significantly cheaper and faster than traditional automated biochemical identification techniques.

Answer 12

5 to 10

Question 13

used to amplify DNA from the unknown pathogen and ESI-TOF MS is used to calculate exact masses of these PCR-generated DNA fragments

Answer 13

PCR

Question 14

The unique purine and pyrimidine nucleotide composition of the PCR-generated fragments can then be compared with a preexisting digital library in order to identify the

Answer 14

unknown pathogen