cc one Flashcards
- The milky appearance of the serum specimen causes falsely elevated bilirubin. Bilirubin is also increased if the sample is hemolyzed.
A. Only 1st statement is true
- Which of the following does not affect ammonia level?
1) Hemolysis
2) Incorrect blood to additive ratio
3) Tobacco exposure
4) Cold transport
5) Prolonged tourniquet application
6) Fasting
C. 4&6
- All of these amino acids have maximum absorbance at 210 nm, except:
a. Threonine
b. Tryptophan
c. Proline
B. Tryptophan
- Which of the following coagulant factors is not
dependent on Vitamin K?
A. Stuart Factor
B. Labile Factor
C. Prothrombin
D. Christmas Factor
B. Labile Factor
- What are the sources of Bilirubin?
a. Hemoglobin
b. Myoglobin
c. Catalase
d. Both A & D
e. All of these
E. All of these
- The principle of Biuret Method is based on?
a. Oxidation of phenolic amino acids of proteins
b. Based on the amount of nitrogen in proteins
c. The reaction of copper with primary amino groups
d. The number of ester bonds
E. None (Based on the number of peptide bonds)
- Slow reacting bilirubin is produced from reduction of biliverdin. What enzyme reduces Biliverdin?
a. Bilirubin reductase
b. Heme oxygenase
c. Biliverdin reductase
d. Cytochrome oxidase
C. Biliverdin Reductase
- Rotor syndrome: Bilirubin transport deficit, ____ : Circulating coagulation inhibitor
a. Gilbert’s Syndrome
b. Dubin-Johnson Syndrome
c. Crigler-Najjar Syndrome
d. Lucey-Driscoll Syndrome
D. Lucey-Driscoll Syndrome
- In Bilirubin transport deficit, non-polar bilirubin is ____, while total bilirubin is ____.
C. Increased, Increased
- Which of the following is/are hepatic enzymes?
1) Aldolase
2) Lactate dehydrogenase
3) Acid phosphatase
4) Gamma glutamyltransferase
D. 2 & 4
- Enzyme employed in ammonia measurement utilizing automated analyzers
a. Urease
b. Lactate dehydrogenase
c. Malate dehydrogenase
d. Glutamate decarboxylase
E. None
- In hemolytic anemia, hemobilirubin is ____, while urobilinogen is ____.
A. Increased, Normal
- Albumin is the most abundant protein in the blood, in serum protein electrophoresis it migrates to the point of origin.
A. Only the first statement is true
- In post hepatic jaundice, polar bilirubin is ____, while non-polar bilirubin is ____.
A. Increased, Normal
- Crigler-Najjar Syndrome is a type of unconjugated hyperbilirubinemia due to?
a. Inability to transport Bilirubin 1 to the liver
b. Presence of circulating UDPGT inhibitor
c. Impaired Bilirubin 2 excretion
d. Absence of conjugating enzymes
D. Absence of conjugating enzymes
- What is the purpose of methanol in Evelyn and Malloy method?
a. To allow measurement of Bilirubin 1
b. Accelerant
c. Both
d. Neither
C. Both
- Diazo A : 0.1% Sulfanilic Acid, Diazo B : ____
a. 1.5% Hydrochloric acid
b. 0.5% Sulfanilic acid
c. 0.5% Sodium nitrite
d. None
C. 0.5% Sodium nitrite
- Ammonia is a neurotoxin leading to comma, urea is synthesized from ammonia through the Krebs Cycle.
A. Only 1st statement is true
- Macdonald Method: ____, Albumin Measurement: Liver Synthetic function
C. Liver Excretory function
- The number one marker for obstructive jaundice?
C. Alkaline Phosphatase
- Kjeldahl method assumes that the nitrogen method of protein is approximately ____?
a. 15.1%
b. 16.1%
c. 15%
d. 16%
D. 16%
- Veronal in serum protein electrophoresis ____ pH of the medium, which causes protein to be ____
B. Increases, negatively charged
- In serum protein electrophoresis, proteins migrate towards the ____?
A. Positively charged electrode
- Urobilinogen is produced by reduction of non-polar bilirubin by intestinal bacteria, measurement of bilirubin utilizes p-phenylenediamine dihydrochloride.
B. Both statements are false (polar, diazo reagent)
- All proteins are synthesized by the liver except for immunoglobulins, Antibodies migrate nearest to the point of origin during SPE
C. Both statements are true
- Plasma is not utilized in total protein measurement as:
a. It can falsely elevate total protein level
b. It has fibrinogen
c. Both are correct
d. Neither
C. Both are correct
- Kernicterus may appear on patients with chronic elevation on which bilirubin?
a. Bilirubin 1
b. Unconjugated bilirubin
c. Water insoluble bilirubin
d. Both b and C
e. E. all of these
E. All of these
- Abnormal bilirubin increased in Dubin-Johnson syndrome
a. Hemobilirubin
b. Delta bilirubin
c. Polar bilirubin bound to albumin
d. Both b & c
e. Both a & b
D. Both b & c
- Which of these dyes will only bind to albumin?
a. Bromcresol green
b. Bromcresol purple
c. Methyl orange
d. All of these
B. Bromcresol purple
- Prehepatic bilirubin is carried by albumin to the liver, in the liver this bilirubin is carried by ligandins.
C. Both statements are true
- Storage temperature of specimen for Urea measurement
D. 2 – 8 degC
- To convert BUN to urea, the value is multiplied to
C. 2.14
- Lloyd’s reagent: ____, Fuller’s earth reagent: aluminum magnesium silicate
B. Sodium aluminum silicate
- The type of azotemia associated with decreased blood delivered to the kidneys
A. Pre-renal azotemia
- What is the normal creatinine to BUN ratio?
B. 1:20
- Uric acid occurs as monosodium urate in plasma at a blood pH of what?
a. 7. 35
b. 7.45
c. 7.40
d. All of these
D. All of these
- ____: Jaffe reagent, Bilirubin: Diazo reagent
a. Urea nitrogen
b. Uric acid
c. Creatinine
d. Ammonia
C. Creatinine
- Arrange the following NPNs in ascending order of abundance
1) Creatinine
2) Creatine
3) Urea
4) Amino acids
5) Uric acid
6) Ammonia
D. 6,2,1,5,4,3
- Major site of reabsorption of essential substances in the nephrons
Proximal convoluted tubules
- Uricase enzyme causes oxidation of uric acid, forming
B. Allantoin
