Mass Spec Flashcards
What is Mass spec?
Gas phase ions are separated and detected according to their mass to charge ratio
What are the components of Mass spec?
Take some time to review the next slide which will show you how to calculate the M/Z value of a compound.
*Assume it will go through a depolarization
Take some time to review the next slide for M/Z value
Assume it goes through polarization
What is High resolution mass spectrometry?
It can measure the MZ value so accurate and it differentiate the differences of small exact mass values
What are the advantages of low resolution mass spec?
Quantifying
What is tandem mass spec?
A technique where we are selective with in terms of what we want to break down. For example if we wanted just detect a certain compound
What is fragmentation?
Where we break down a specific product into different fragments (Example)
What happens when you combine HPLC with MS?
Essentially you get it where we can clean out a blood sample via LC for example. Then using selecting process of mass spec we can break down and select for precursor. During the selection process we can then get the product to analyze
Case study review:
- Isolation of the contents
a. Wash the Ampoule with methanol, cheap, good solvent that dissolves everything
b. Run direct into mass spec
c. We can run MS-MS, of either of the compounds
d. Compare the isoelectric point
Utilize mass spectrometry
How does analysis based on fuel cell technology work? (Breathalyzer)
If any alcohol is presnet in the exhaled air the alcohol is converted into acetic acid and the hydrogen ion and electrons on the top surface move to the platinum oxide which is then detected on the bottom surface
How does Airport security work?
What is Ion mobility spectrometry-MS?
Very similar, but just allows ofr further analyzation of contents. Isolate and scan ions
Can genes be analyzed by mass spec?
No I dont think?
What are the applications of metabolomics?
Define Selectivity/specificity
Define Recovery
Define Accuracy
Define Precision
Define Linearity
Define Range
Define limit of detection
Define Limit of quantitation
Define Robustness
Define stability
