Antibodies Flashcards

1
Q

What are the specific functions of antibodies in cancer therapy?

A

Tumor specific IgG
Angiogenesis inhibition
Checkpoint blockade
Radioimmunoptherapy
Antibody-drug conjugate therapy
Bispecific antibody therapy
CAR T Cells

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2
Q

How big is an antibody generally?

A

150kDa

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3
Q

What are the components of a antidbody

A

Heavy chain light chain, where the head is Varaiable and the tail is constant

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4
Q

Where does the c portion of antibodies bind to?

A

FC receptors

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5
Q

What is the Fab portion of an antibody?

A

Antigen binding

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6
Q

What does is mean to be a heteroetramer?

A
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7
Q

What makes an antibody specific?

A

CDR loops

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8
Q

How is antibody diversity generated?

A
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9
Q

What is the number of possible heavy and light chain combinations?

A

2.64x106

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10
Q

How do we make antibodies for protiens of interest?

A

Hybridoma method, Xenomouse technology, phage display and selection

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11
Q

What are the 4 methods for developing antibodies?

A

Mouse hybridoma, Phage Display, Transgenic mouse, single B cell

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12
Q

What are chimeric antibodies?

A

Where portions are mouse specific (Such as the antigen specific region)_ and the tail region is human specific so that we can use it in humans

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13
Q

What cells make antibodies?

A

B Cells

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14
Q

What portions are compatible with page display

A

Only scFv and VH/VHH

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15
Q

What is the success rate from phase I study to FDA approval for antibody treatment/development?

A

About 25% where small Molecules drugs are about 7%

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16
Q

What % of antibodies are targeted against the 10 top candidates?

A

45

17
Q

What are the top 10 mAB targets?

A
18
Q

What is the Bevacizumab?

A

Targets VEGFA which inhibits angiogenesis and is used for age related macular degeneration and several acncers

19
Q

What is controversy between bevacizumab and lucentis?

A

Lucentis costs alot more, but moth have equal efficacy

20
Q

What can mutations in Fc region of antibody tdo/

A

It can alter the binding affinity to Fc gamma receptors and decrease antibody effector functions as desired

21
Q

What has engineering the Fc fragment done to antibody therapies?

A

Increased half life by preventing degredatation of the lysosomes

22
Q

What are bispecific antibodies?

A
23
Q

What is Biparatopic binding?

A

Therefore better for drug conjugation

24
Q

What is a quadroma/

A

All 4 main components of antibody are different therefore leading to 10 diferent products

25
Q

What is Knob in holes technology?

A

Mutations prevent/reduce homodimerization of heavyc cahin and light chain crossover can still happen

26
Q

WHat is Cross MAB technology?

A

Enables purificaiton of correctly paired antibodies, light chain crossover is prevented

27
Q

What do bispecific antibodies allow ofr?

A
28
Q

(DONT BOTHER) What are the advanatages/disadvantages of alternativer scaffolds?

A
29
Q
A