Antibodies Flashcards
What are the specific functions of antibodies in cancer therapy?
Tumor specific IgG
Angiogenesis inhibition
Checkpoint blockade
Radioimmunoptherapy
Antibody-drug conjugate therapy
Bispecific antibody therapy
CAR T Cells
How big is an antibody generally?
150kDa
What are the components of a antidbody
Heavy chain light chain, where the head is Varaiable and the tail is constant
Where does the c portion of antibodies bind to?
FC receptors
What is the Fab portion of an antibody?
Antigen binding
What does is mean to be a heteroetramer?
What makes an antibody specific?
CDR loops
How is antibody diversity generated?
What is the number of possible heavy and light chain combinations?
2.64x106
How do we make antibodies for protiens of interest?
Hybridoma method, Xenomouse technology, phage display and selection
What are the 4 methods for developing antibodies?
Mouse hybridoma, Phage Display, Transgenic mouse, single B cell
What are chimeric antibodies?
Where portions are mouse specific (Such as the antigen specific region)_ and the tail region is human specific so that we can use it in humans
What cells make antibodies?
B Cells
What portions are compatible with page display
Only scFv and VH/VHH
What is the success rate from phase I study to FDA approval for antibody treatment/development?
About 25% where small Molecules drugs are about 7%