Making Monoclonal Antibodies Steps Flashcards
1
Q
What is the first step in making monoclonal antibodies?
A
- A mouse is injected with a foreign antigen to begin the production of antibodies by lymphocytes
2
Q
What is the second step in making monoclonal antibodies?
A
Lymphocytes are isolated usually by removing the mouses spleen
3
Q
What is step 3 of making monoclonal antibodies?
A
A fast dividing Tumor cell is fused with the lymphocyte
4
Q
What is step 4 in making monoclonal antibodies?
A
A hybridoma cell is formed- a fast diving cell capable of making the right antibody
5
Q
What happens in step 5 of making monoclonal antibodies?
A
The hybridoma divides many times
6
Q
What happens in step 6 of making monoclonal antibodies?
A
The antibody is collected and can be used to bind with the specific antigen
