Major Histocompatibility Complex: Structure and Function- Dr. hudson

Question 1

Can you transfer the product of B cells (serum antibodies) to a non infected (naive) mouse to protect it from a virus?

Answer 1

Yes, it is called passive immunization. Gamma-globulin (IgG) injection for HIV patients, HBsIgs for hepatitis patients. Expensive because antibodies have half life of 30 days so need to harvest often

Question 2

If you transfer t cells from infected mouse to syngeneic (identical) mouse, they will be protected. If you transfer them to a healthy allogeneic (different strain) mouse, they will not be. Why?

Answer 2

Because t cells always recognize foreign antigen in the context of self.

Question 3

HLA-A, -B, and -C are the heavy chains of _ molecules

Answer 3

class 1

Question 4

Six classical gene products.. heterodimers of alpha and beta chains of HLA-DR, -DQ, and -DP are gene products of _ molecules

Answer 4

class 2 MHC

Question 5

How do class 1 MHC molecules allow for diverse recognition?

Think HLA-A, B, C

Answer 5

They are polygenic- There is HLA-A, B- and C.

They are codominant and polymorphic, which means there is one HLA-A from your mom and one from your dad.

So your genotype can be…

HLA-A2, HLA-A9, HLA-B27, HLA B330, HLA C4, HLAC23 for example

Question 6

Three classical class 1 MHC gene products

Answer 6

HLA-A, HLA-B, HLA-C

Question 7

In a class 1 MHC structure, the _ and _ domains of the molecule are what contain the polymorphisms in the population

Answer 7

alpha-1 and alpha-2 peptide binding

Question 8

In class 2 mhc molecules, _ (number) chains span the membrane,

in class 1 mhc molecules, _ chain spans the membrane

Answer 8

two, one

Question 9

Which MHC class 2 gene product has the most polymorphisms in the population?

alpha or beta chain?

Answer 9

The beta chain

Question 10

In class 2 molecules, both chains span the membrane, but the _ and _ domains of the molecule are the peptide binding domains

Answer 10

A1 and B1

Question 11

What are the four domains of the MHC class 2 molecule?

Answer 11

A1- protein binding B1- protein binding

A2- membrane spanning, B2, membrane spanning. these two contain immunoglobulin fold

Question 12

Polymorphisms in Class 1 MHC proteins occur in the _ and _ domains

Answer 12

a1 and a2 peptide binding domains

Question 13

Hepatitis b non responders have which two haplotypes?

Answer 13

HLA-DR7 and HLA-DR3

Homozygotes for these two haplotypes are found almost exclusively in the non responder (NR) population

It’s a problem w/ t cell response not with antigen presenting cell

Question 14

Ankylosing spondylitis, inflammatory possibly autoimmune disease of verterbral joints. Tissue type with _ haplotype is seen in 93% of individuals with AS

Answer 14

HLA B27