Magnification and Quantitive Units Flashcards

1
Q

Equation: Magnification

A

Image size
—————
Actual size

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2
Q

What is magnification

A

The process of enlarging an image

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3
Q

What is resolution?

A

The ability to distinguish between two points as separate structures (level of detail)

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4
Q

What is a compound microscope?

A

A microscope that uses multiple lenses

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5
Q

What is the maximum magnification of a light microscope?

A

x2000

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6
Q

How do you calculated the overall magnification of a microscope?

A

Overall magnification = eyepiece magnification x objective magnification

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7
Q

What is a light microscope?

A

A type of microscope that uses lenses to magnify an image

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8
Q

What is an electron microscope?

A

A microscope that uses beams of electrons to form an image

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9
Q

What are the differences between light and electron microscopes? (3)

A
  • Light microscopes use light, electron microscopes use electrons
  • Electron microscopes have a higher resolution
  • Electron microscopes have a higher magnification
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10
Q

How have changes in microscopes lead to us seeing things in the detail we do today?

A
  • Compound microscopes was the first type invented
  • Single lens microscopes then invented
  • Optical quality of light microscopes increased over time but reached a point where magnification couldn’t get higher
  • First electron microscope in 1931
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11
Q

What are the disadvantages of electron microscopes?

A
  • Living cells cannot be observed since they have to be placed in a vacuum
  • Expensive
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12
Q

What are the two types of electron microscopes?

A
  • A Transmission Electron Microscope (TEM)
  • A Scanning Electron Microscope (SEM)
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13
Q

What is one mm in metres?

A

1x10-3

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14
Q

What is one μm in metres?

A

1x10-6

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15
Q

What is one nm in metres?

A

1x10-9

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16
Q

What is one pm in metres?

A

1x10-12

17
Q

What is the stage on a microscope?

A

Where the slide is placed

18
Q

Where is a slide placed on a microscope?

A

On the stage

19
Q

What holds a slide in place on a microscope?

A

Stage clips

20
Q

What is used to shine light onto a microscope slide?

A

Either a lamp or a mirror

21
Q

What are the two types of microscope lens?

A
  • Objective lens
  • Eyepiece lens
22
Q

What are the names of the lenses above the stage on a microscope?

A

Objective lenses

23
Q

What are the two parts of the microscope that improve the resolution of the image?

A
  • Coarse focussing wheel
  • Fine focussing wheel
24
Q

What is the coarse focussing wheel on a microscope for?

A

Positioning the objective lens so it almost touches the slide

25
Q

What is the fine focussing wheel on a microscope for?

A

Adjusting the resolution of the image when viewing the slide through the eyepiece lens

26
Q

Describe the preparation of a microscope slide

A
  • Put a drop of water on a microscope slide
  • Put the specimen on the water
  • Add a drop of a stain eg. iodine
  • Place coverslip on top