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BOTANY LAB > M1 & M2: MICROSCOPE & CALCULATIONS > Flashcards

M1 & M2: MICROSCOPE & CALCULATIONS Flashcards

1
Q

an instrument for viewing what is small

A

MICROSCOPIUM

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2
Q

the word microscope came from the greek word

A

SKOPION “means of viewing”

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3
Q

WHAT DOES SKOPION MEANS

A

MEANS OF VIEWING

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4
Q

SKOPION came from the word

A

SKOPEIN “look at”

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5
Q

WHAT DOES SKOPEIN MEANS

A

LOOK AT

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6
Q

CONTAINS THE OCULAR LENS which you will be looking through to see the magnified specimens with magnification raging from 5X to 30X, but 10X or 15X is the most common in use.

A

EYEPIECE

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7
Q

provides a RE-MAGNIFIED IMAGE to see when light enters through the objective lens.

A

OCULAR LENS

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8
Q

It CONNECTS the EYEPIECE AND OCULAR LENS to the OBJECTIVE LENSES

A

EYEPIECE TUBE

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9
Q

It connects the EYEPIECE TUBE TO THE BASE where you should hold when carrying the microscope.

A

MICROSCOPE ARM

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10
Q

It provides STABILITY AND SUPPORT for the microscope in its upright position. Typically, it HOLDS THE SOURCE OF LIGHT or illuminator.

A

MICROSCOPE BASE

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11
Q

It is a LIGHT SOURCE which can come in a form of a built-in, low voltage illuminator light, or a mirror that reflects an external light source like sunlight.

A

ILLUMINATOR

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12
Q

It serves as the PLATFORM FOR SLIDES. Some has mechanical stage with adjustment knobs that allow movement of slides to achieve more precise positioning.

A

STAGE

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13
Q

HOLD THE SPECIMEN IN PLACE on either side.

A

STAGE CLIPS

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14
Q

It CONTAINS THE OBJECTIVE LENSES. You can rotate this part in switching objective lenses and adjust the magnification power.

A

NOSEPIECE

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15
Q

Generally, microscope feature three or four _______, with magnification levels ranging 4X to 100X. _____ are combining with the eyepiece lens to INCREASE MAGNIFICATION LEVELS. ______ are the lenses that PROTRUDE DOWNWARD over the specimen.

A

OBJECTIVE LENSES

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16
Q

magnification level of SCANNING lens

A

4X

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17
Q

magnification level of LPO lens

A

10X

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18
Q

magnification level of HPO lens

A

40X

19
Q

magnification level of OIO lens

A

100X

20
Q

It PREVENTS users from MOVING OBJECTIVES TOO CLOSE TO THE SLIDE

A

RACK STOP

21
Q

used to bring the specimen into INITIAL FOCUS – visible but not sharp

A

COARSE ADJUSTMENT KNOB

22
Q

turned to bring the specimen into SHARP FOCUS.

A

FINE ADJUSTMENT KNOB

23
Q

adjusts the distance between the stage and the lens

A

KNOBS

24
Q

CONCENTRATES THE LIGHT on the specimen

A

CONDENSER

25
Q

a SMALL MOVABLE LEVER is adjusted that REGULATES THE ENTRY OF LIGHT.

A

DIAPHRAGM

26
Q

the MEASURE for the ABILITY TO TELL TWO POINTS APART. It describes whether two adjoining points can still be perceived as separate.

A

POWER OF RESOLUTION

27
Q

the product of Vobjective X Vocular

A

MAGNIFICATION OF A MICROSCOPE

28
Q

the SINE OF HALF THE ANGLE of the cone of light from each point of the object that can be accepted by the objective multiplied by the index of refraction of the medium in which the object is immersed.

A

NUMERICAL APERTURE

29
Q

refers to the DISTANCE FROM THE COVER GLASS to the nearest point of the objective.

A

WORKING DISTANCE

30
Q

refers to the DISTANCE BETWEEN THE UPPER AND LOWER LIMITS OF SHARPNESS in the image formed by an optical system.

A

FOCAL DEPTH

31
Q

This is a number that REPRESENTS THE DIAMETER IN MM of the image of the field diaphragm that is formed by the lens in front of it.

A

FIELD NUMBER

32
Q

The ACTUAL SIZE OF THE FIELD OF VIEW in mm on the object surface

A

FIELD OF VIEW DIAMETER

33
Q

Magnifying an object WITHOUT GOOD RESOLUTION is called _________

A

EMPTY MAGNIFICATION

34
Q

FORMULA FOR TOTAL MAGNIFICATION

A

Vobjective x Vocular

35
Q

magnification of OCULAR LENS

A

10X

36
Q

FOV using EYEPIECE only

A

magnification / field number (20)

***expressed in mm

37
Q

FOV using EYEPIECE & OBJECTIVE LENS

A

Vocular x Vobjective = ans

field number (20) / ans

***expressed in mm

38
Q

refractive index n of air

A

1

39
Q

refractive index n of water

A

1.33

40
Q

refractive index n of glycerin

A

1.47

41
Q

refractive index n of oil

A

1.518

42
Q

refractive index n of oil 444067

A

1.66

43
Q

formula for ACTUAL SIZE of the cell

A

dFOV (usually 2mm) / no. of cells na nakikita within the FOV

**if naka micrometer, conver to mm
**if naka mm, convert to micrometer
***usually expressed in mm (depends on the given intsruction)

44
Q

formula for DRAWING MAGNIFICATION

A

drawing size / actual size in um

***if naka mm, convert to um

BOTANY LAB (12 decks)

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