Locomotor CPG control in mammals Flashcards
where are CPGs localised
in the ventral horn of the spinal cord, predominantly in the upper lumbar regions
what are two methods of investigating CPG neurons
staining
gene transcription
what are the methods of genetically studying spinal interneurons
knockout and cre-lox system
what is a protein expressed in spinal cord for genetic methods
green fluorescent protein (GFP)
what is genetic knockout?
removal of gene using homologous recombination
what is cre-lox system
cre recomination mediates recombination between loxP sites
what is conditional expression
tissue specific cre protein in one mouse(for example), breed ad anything downstream stops etc
what is the most likely source of rhythm generation
ipsilaterally projecting glutaminergic excitatory internueorns
which internuerons generate putative rhythm
HB9 expressing interneurons- glutaminergic and coupled by gap juncitons
which interneurons control left right coordination in mammals
Dbx-1 (V0)
which interneurons control locomotor frequency in mammals
V1
ipsilaterlaly projecting and inhibitory
