Ligninger og formler

Question 1

Ændring i den frie energi (∆G)

Answer 1

∆G°= ∆H - T∆S

Question 2

Associationskonstanten

Answer 2

Ka = [PL] / [P] x [L]

Question 3

Dissociationskonstanten

Answer 3

K_D = [L] x [P]/ [PL]

Question 4

Relationen mellem dissociationskonstanten og associationskonstanten

Answer 4

Ka = 1/K_D

Question 5

Bindingsenergien

Answer 5

∆G°= - RT ln K_eq

Question 6

Lambert Beer’s lov

Answer 6

A = log (I_0/I) = 𝜀 x c x l

Question 7

Plancks ligning

Answer 7

E = h · f = (h ·c) / 𝜆

Question 8

Proteinstabiliteten i denaturant

Answer 8

∆G°(N-D) = ∆G°(N-D)^H2O + m ·[den]

Question 9

Proteinstabiliteten ved ligevægt

Answer 9

∆G°(N-D)^H2O = - |m| x Cm

Cm: [den]_50%

Question 10

Forskel i stabillitet mellem WT og MUT

Answer 10

∆∆G°(MUT-WT) = ∆G°_WT - ∆G°_MUT

∆∆G°(MUT-WT) 0, WT stabil

Question 11

Beregning af pI

Answer 11

pI = 1/2 (pK_1 + pK_2)

Question 12

Henderson-Hasselbach ligningen

Answer 12

pH = pKa + log ( [B]/ [S] )

Question 13

Michaelis-Mentens ligning

Answer 13

V_0 = (Vmax · [S]) / (Km + [S])

Question 14

Lineweaver-Burk ligningen

Answer 14

1/V_0 = Km/Vmax ·1/[S]+ 1/Vmax

Question 15

Km

Answer 15

Km = (K_2 + K_-1) / K_1

Question 16

Kd

Answer 16

Kd = K_-1 / K_1

Question 17

kcat

Answer 17

kcat = Vmax / [Et]

Question 18

Vmax

Answer 18

Vmax = kcat ·[Et]

Question 19

Specificitet konstanten

Answer 19

kcat / Km

Question 20

Varmekapacitet

Answer 20

Cp = ∂H/∂T = T∂s/∂T