Lesson 1.2

Question 1

STERILIZATION OF HIGH QUALITY PLASTICWARE
1. Autoclaving at _____C at ___psi for ___ mins
2. Chemical sterilization
3. Gas sterilization

Answer 1

1. 121°C at 15 psi for 15-20 mins
2. Benzalkonium chloride
3. Ethylene oxide

Question 2

According to Graduation/ Purpose
A. Transfer Pipets
1. used to transfer aqueous solutions & non-viscous samples
- self or blow?
- read at what meniscus
- where is the bulb
2. used to biologic fluids having viscosity greater than water
- self or blow?
- read at what meniscus
- where is the bulb
3. used to transfer solutions or biologic fluids without consideration of a specific volume
4. macro or micro pipette

Answer 2

1. Volumetric or Transfer pipet
   - self
   - lower
   - center
2. Ostwald-Folin pipet
   - blow
   - upper
   - near at tip
3. Pasteur pipette
4. Automatic

Question 3

According to Graduation/ Purpose
B. Graduated/ Measuring Pipeta
1. calibrated between two marks; deliver between their calibration marks
- self or blow
- orifice?
- graduation should ___ the tip
2. another one
- self or blow
- orifice?
- graduation should ___ the tip

Answer 3

1. Mohr
   - self
   - smaller
   - graduation should not touch the tip
2. Serological
   - blow
   - larger
   - graduation should touch the tip

Question 4

According to Use
A. holds the particular volume but does not dispense the exact volume
- requires ____
- calibrated with ____
- usually ___ which are expressed in ____
B. delivers the exact volume indicated; Calibrated for the volume delivered
- fluid is ___ to flow freely with the pipet tip touching the inner wall of receiving vessel

Answer 4

A. TC
- rinsing
- mercury
- micropipette; microliter
B. TD
- allowed

Question 5

Three general types of Automatic:
1. relies on a piston for suction creation to draw the sample into a disposable tip
2. operates by moving the piston in the tip or barrel
- sample enters directly upon contact without ____
- is there any need to replace tip?
3. obtain the liquid from a common reservoir and dispense it repeatedly; combines sampling & dispensing functions

Answer 5

1. Air-displacement
2. Positive displacement
   - air interference
   - no
3. Dilutor/Dispenser pipets

Question 6

Centrifugation
1. RCF in grams ____
2. or use _____
3. RPM = ____ or ____

Answer 6

1. 1.118 x 10-5 x r x (rpm)^2
2. nanogram
3. tachometer or strobe light

Question 7

Types of Centrifugation
1. tubes placed in the cups of the rotor assume a horizontal plane when the rotor is in motion and vertical position when at rest
- aka
- when at rest
- when it moves
2. particles are driven outside and bottom of the tube and the surface of the sediment packs against the side and bottom of the tube and the surface of the sediment is parallel to the shaft of the centrifuge
- aka
- tubes are held at a fixed angle from ___ degrees to the vertical axis of rotation
3. High-speed centrifuge; mainly fixed angle rotor
- requires ____
- tube from tip to bottom: __>__>__>__
- largest but lightest
- smallest but heaviest

Answer 7

1. Horizontal-head
   - swinging-bucket centrifuge
   - vertical
   - horizontal
2. Angle-head
   - fixed angle centrifuge
   - 25-40
3. Ultracentrifuge
   - refrigerated chamber
   - CM>VLDL>LDL>HDL
   - chylomicrons
   - hdl

Question 8

WASHING SOLUTIONS:
1. Presoaking: ___ or ____ (dilution)
- serves as
- for HIV _____; for ____HB
2. Cleaning: ___
- this contains ___ and _____ or _____
3. Final rinsing use ___ or ____
- for washing glasswares
4. Sterilization
- __ heat over temp
- temp ____ or ____
- duration

Answer 8

1. soapy water or dilute bleach (1:5 or 1:10)
   - decontamination process
   - HIV: 2mins; HB: 10mins
2. acid dichromate
   - potassium dichromate and sulfuric acid (H2SO4) or nitric acid (HNO3)
3. Type I or Type II
   - Type III
4. • Dry heat
   • > 140C or 160-180C
   • 1.5 to 2hrs