1 Flashcards
- seeks to understand the physiologic and biochemical processes occurring in
normal and abnormal states - analyses performed on body fluids or tissues to provide important information for the diagnosis and treatment of disease
- Fundamental Science
- Applied Science
LABORATORY REAGENTS: Chemicals
- of high purity and suitable for most analytical lab procedures
- meet specifications set by the _______
- includes (3) - used to manufacture drugs but not for lab
- less pure grade; chemical prep are not uniform
- aka - lowest quality and should not be used for analytical work
- Analytical Grade
- American Chemical Society
- al laboratory procedures
- Includes - United States Pharmacopeia (USP) & National Formulary (NF)
- Chemically pure
- Pure Grade Chemicals - Technical/ Commercial grade
LABORATORY REAGENTS: Standards
- highly purified chemicals that can be measured directly to produce a substance of exact known
concentration
- used for standardization of solutions of ____ strength
- stable and can be dried, preferably at ____˚C, without a change in composition;
- not _____ - used as primary standard materials in the
clinical laboratory
- certified by ____
- NIST
- IRMM - of lower purity with concentration determined by comparison with a primary standard
- PRIMARY STNDARD
- UNKNOWN STRENGTH
- 104-110C
- HYGROSCOPIC - STANDARD REFERENCE MATERILS
- NATIONAL BUREAU OF STANDARDS
- National Institute of Standards and Technology
- Institute for Reference Materials and Measurements - SECONDARY STANDARD
GRADES OF WATER
- used in test methods requiring minimum interference and maximum precision and accuracy; tissue or cell culture
- analysis of electrolytes - acceptable for most analytic procedures; hema cc immuno
- urinalysis, parasitology, histology acceptable for washing glasswares
- Type I
- ultramicro analysis - Type II
- Type III
PREPARATION OF REAGENT GRADE WATER
____: purified to remove almost all organic materials
- Water is boiled and vaporized; Each distillation cycle removes impurities
- Removes 98% of particulate matter
- ____: removes organic materials and chlorine
- ______ & _____: remove particulate matters, microorganisms, pyrogens & endotoxins - Uses pressure to force water thru
semipermeable membrane - ____: some or all ions removed
- produced using either ___ or ____ _____
Distilled water
- Distillation
- Filtration
- activated carbon
- Ultrafiltration and Nanofiltration - Reverse Osmosis
- Deionization
- anion or cation exchange resin
FACTORS DETERMINING THERMAL DURABILITY:
1. temperature resulting to deformation due to heat stress
2. °T at w/c glass is heated in order to prevent brittleness
3. dimension change w/ °T (ideally, it should be low)
- Strain point
- Annealing point
- Coefficient of Expansion
PROPERTIES OF GLASS:
1. dependent on silicate anion content (greater amount – more durable)
2. dependent on boron oxide, nickel & ferric ion content
3. dependent on ferric ion content
- Breakability
- Thermal Durability
- Transparency
HIGH THERMAL RESISTANCE
- With high degree of thermal resistance ( ___C)
- Strengthened chemically rather than thermally;
- ___x stronger than borosilicate glass but less thermally resistant
- resists some clouding and scratching
- Borosilicate w/ Low Alkaline content (510C)
- Alumina-silicate glasswares
- 6x
- Alkali resistant
ALUMINA-SILICATE GLASSWARES
- with good thermal endurance (____C)
- __% silica - with poor heat resistance but has high resistance to alkali
- aka - thermally resistant and with a red or amber color
- cheapest and with poor resistance to high temperatures
- ___ glass composed of oxides of ___, ____, ____ - ___ tolerances according to NIST: high ____ or ______ glass
- High Silica Glasswares
- 96% silica - Boron-free glass
- Soft glasswares - Low-Actinic glass
- Flint glass
- soda lime glass: Silicon, Calcium, Sodium - Class A: high thermal borosilicate or aluminosilicate glass
SPECIAL GLASSWARES
1. has metallic oxides, for filters and light bulbs
2. has a thin metallic oxide permanently fire bonded to its structure
3. made of soda lime, lead and borosilicate
4. with high thermal resistance chemical stability and corrosion resistance
5. made of soda lime and lead
- Colored and Opal glassses
- Coated glass
- Optical glass
- Glass ceramics
- Radiation-Absorbing Glass