Lecture 9 : Energetics of Protein and Action Flashcards
1
Q
Types of enzymes
A
- Hydrolases (cleavages, proteases)
- Kinases (addition / removal of phosphates)
- Isomerases (rearrangements)
2
Q
Exergonic reaction
A
Spontaneous, gibbs energy < 0
3
Q
Why is the induced fit hypothesis important
A
- Conformational changes in the protein’s active site allow for compensation between ∆H and ∆S to minimise ∆G
4
Q
How to let an endergonic reaction happen
A
- Couple an endergonic reaction with an exergonic one
5
Q
Assumptions of Michaelis Mentin shit
A
- Assume reverse reaction is negligible
- Assume only 1 ES exists
- Assume S > E
6
Q
What is Vmax
A
When enzyme is fully occupied by substrate
7
Q
What is Kcat
A
Measure of enzyme efficiency
8
Q
What is V0 and Km
A
9
Q
What is the michaelis menten equation
A
10
Q
Summary
A
