Lecture 5 : Protein Interactions Flashcards
1
Q
What can proteins interact with?
A
- Small ligands (metabolites, ions, metal ions, neurotransmitters, antigens)
- Other proteins (hormones, receptors, CSM proteins)
- Lipids in membranes
- DNA (chromatin, polymerase enzymes) and RNA
- Sugars and carbs
2
Q
What is calmodulin?
A
- Main censor of Ca2+
- Activated by Ca2+
- Binds to lots of targets to carry out physiological responses
- Eg. Membrane receptors, enzymes, etc
3
Q
How do they interact?
A
- Mostly with non-covalent interactions
- Ionic, hydrogen bonding, van der waals
- Rarely by disulphide bridges
4
Q
What can increase the chances of proteins forming complexes?
A
- Conc of protein and substrate
- Competition from alternative substrates
- Matching non-covalent interactions
5
Q
How can interactions between proteins be regulated?
A
- Altering pH
- Competitive inhibition
- Post transcriptional modifications
