Lecture 7 : Measuring Protein Activity by Spectrometry Flashcards
1
Q
What is spectroscopy?
A
The study of absorption and emission of EM radiation by matter
2
Q
What can spectroscopy help us analyse?
A
- Is the protein bound or free? (hormones)
- Which protein is present
- How concentrated is the protein
- How effective is it
3
Q
What determines the energy of a wave?
A
- Its wavelength and frequency
- Lower wavelength, higher frequency
4
Q
What is UV-visible spectroscopy
A
- Mainly in bio
- UV delocalises electrons
- Atoms absorb light at specific wavelength
- At certain wavelengths, how much light absorbed is used to estimate concentration of sample
- Absorption spectrum gives fingerprint of bio molecule
5
Q
Beer lambert relationship
A
Molar absorption is a measure of how well a substance absorbs light
6
Q
What is the solvent effect
A
7
Q
Summary
A
