Lecture 8 - Transport Flashcards

1
Q

Membrane transport (passive vs active)

A

Passive transport:
Substances move down concentration gradient (high to low)
Use no energy

Active transport:
Substances move against a concentration gradient (low to high)
Energy required

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2
Q

Diffusion

A

Movement of molecules DOWN a concentration gradient

Influenced by:
Mass of molecule
Temp. of molecule
Steepness of gradient
Surface area
Distance to diffuse

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3
Q

Simple diffusion

A

Diffusion through membrane without channel or carrier
Only done by lipids or very small molecules

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4
Q

Facilitated diffusion

A

Sugars, amino acids or ions bind to protein carriers in membrane or move through channels

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5
Q

Osmosis

A

Movement of water through a selectively permeable membrane
Solute molecules cannot cross

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6
Q

Autophagy

A

“Self eating” cell digests its own structure

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7
Q

Autolysis

A

Breakdown of cells during own enzymatic action

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8
Q

Auqaporins

A

Integral membrane proteins that act as a water channel during osmosis

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9
Q

Tonicity

A

Refers to the ability of a solution to change shape/tone depending on volume of water

Isotonic- same solute concentration
Hypertonic- high solute (crenate)
Hypotonic low solute (lyse)

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10
Q

Active transport processes

A

Energy required because:
Solute too large for channels
Solute not lipid soluble
Solute not able to move down a gradient

Two major active membrane transport processes:
Active transport
Vesicular transport

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11
Q

Carrier proteins

A

Bind specifically and reversibly with substance being moved

Anti-porters- Transport one substance into cell while transporting a different substance out of cell

Symporters- transport two different substances in the same direction

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12
Q

Primary vs secondary active transport

A

Primary active transport:
Required energy comes directly from ATP hydrolysis
Shape change causes solutes to be pumped across the membrane

Secondary active transport:
Required energy comes indirectly from ionic gradients created by primary active

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13
Q

Membrane Potential

A

A membrane is a voltage across the cell membrane that occurs due to separation of oppositely charged particles
Inside of the membrane is more negatively charged than the outside

Resting membrane potential:
Determined by concentration gradient of potassium ions that move freely out of cell down gradient, but also move into cell along electrical gradient

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14
Q

Vesicular transport (endocytosis, exocytosis, transcytosis, vesicular trafficking)

A

Vesicular transport: Uses membrane is vesicles to transport large particles, macromolecules and fluids across the membrane or within cell, this requires energy

Endocytosis- is transport into the cell
Exocytosis- Is transport out of the cell
Transcytosis- Is transport into, across and out of the cell
Vesicular trafficking- is transport from one area for organelle in a cell to another

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15
Q

Phagocytosis (cell eating)

A

Form of endocytosis in which large, solid materials are brought into cell

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16
Q

Pinocytosis (cell drinking)

A

A form of Endocytosis that takes a small volume of extracellular fluids into the cell
No receptor used, process is non-specific

17
Q

Receptor mediated endocytosis

A

Allows cells to concentrate molecules found in small amounts in extracellular fluids
Because it uses receptors, it is HIGHLY SPECIFIC

many cells have receptors embedded in cathrin-coated pits
Enzymes,lipids, iron and insulin taken in this way
Some viruses- diphtheria and cholera toxins trick cells and enter this way

Caveolae are slightly different from clathrin, but still capture specific molecules (folic acid and tetanus toxin) and use transcytosis

18
Q

Exocytosis

A

Type of vesicular transport that moves molecules out of cell in a simple vesicle, often used for secretion or removal of waste from the cell

Protein of vesicle —> vSNARE fuses with tSNARE Proteins on membrane, triggers Exocytosis

Exocytosis can expel hormones,neurotransmitters, mucous etc.