Lecture 7 (Cut off for Exam 2) Flashcards
Third Line Defenses
Two Important Adaptive Immunity Characterisitcs
Pathogen specific and memory based.
Cellular Immunity
- Mediated by T-cells
- Target and kills cells infected and intracellular pathogen
Humoral Immunity
- Antibodies produced by B-cells
- Target extracellular pathogens
Immune Cell Development
Lymphoid Stem Cell»_space; Small Lymphocyte»_space; T-Cell / B-Cell (latter goes into plasma cells)
Cells Involved in Adaptive Immunity Activation (6)
- Macrophage and dendritic cells
- Collectors and informers
- Helper T-cells
- Cytotoxic T-Cells
- B-Cells
- Memory B & T-Cells
Collectors & Informers
- ingest and kill microbes
- antigen presenting and processing
- activation of adaptive immunity
Helper T-Cells
- Central Orchestrators of humoral and cellular immunity
- CD4 markers on surface
- Direct adaptive immunity
- Interact with APCs using MHCII
Cytotoxic T-Cells
- Assassins
- CD8 markers on surface
- Discriminately kill infect host cells (intracellular pathogens)
- Interact with infected cells using MHCI
B-Cells
- Antibody factories
- Humoral immunity
Memory Cells
- Provide long term immunity
- Programmed for faster and stronger reactions
Antigens
- Antibody Generator
- Activates specific adaptive immunity
- Different from PAMP (specificity)
- Also known as immunogens
Bacterial examples of Antigens
Capsule, fimbriae, flagella, LPS, cell wall, toxins
Viral Examples of Antigens
Capsid, spikes, envelopes
Epitopes
Smallest “recognizable” part of antigen that reacts with T-cells and antibodies. Each antigen has multiple epitopes on it.
Antigenicity Factors (3)
- Molecular class
- Shape
- Size
Molecular Class + Antigenicity
- Proteins = most antigenic
- Carbs = least antigenic
- Lipids/nucleic acids = not antigenic usually
Shapes + Antigenicity
- 3D shape = important
- Provides epitopes
Size + Antigenicity
- Larger molecules = more antigenic
- Haplens = too small to be antigenic on their own
Proteins + Antigenicity
Ex: fimbriae, flagella
- Cellular and humoral response
- Longest lasting immune response
- Programmed memory
Carbohydrates + Antigenicity
Ex: Capsule
- Only humoral response
- Weaker and shorter immune response
- Lacks programmed memory
Lymphocyte Receptors
-Surface molecules that bind antigen epitopes
-Provide specificity of immune response
Ex: B-Cell and T-Cell receptors
B-Cell Receptors
- Recognize many molecule types
- Soluble or pathogen-associated
- Doesn’t undergo phagocytosis, internalizes epitopes after they bind to B-cell receptors
T-Cell Receptors
- Recognize proteins only
- Require antigen presentation
Major Hisocompatibility Complex
- Aka MHC, examples = MHC I & MHC II
- Cell surface glycoprotein molecules
- On surface of all nucleated cells
MHC I
- All nucleated cells have it
- Presents normal, abnormal, and foreign antigens
- Presents to cytotoxic T-cells and NK cells
MHC II
- Macrophages, dendritic cells, & B-Cells has receptor
- Presents only abnormal or foreign antigens
- Presents to helper T-cells
Antigen Presenting Cells
- All nucleated cells present antigens with MHC-I
- Professional phagocytes = macrophages, dendritic cels
- APCs present with MHC II to activate helper T-Cells
T-Cell Production
- Produced from multi-potent stem cell
- Differentiate into lymphoblasts in red marrow
T-Cell Maturation
- Lymphoblasts enter blood stream
- Travel to thymus for maturation over 3 phases
- Immature in thymus = thymocytes
Phase I T-Cell Maturation
- Negative selection against thymocytes with defective T-cell receptor (TCR)
- Forces apoptosis to occur (programmed cell death)