lecture 6 thermo

Question 1

For a spontaneous reaction delta G must be what?

Answer 1

<0 (-VE) (less than 0)

Question 2

For a spontaneous reaction delta G must be what?

Answer 2

<0 (-VE) (less than 0)

Question 3

What does > mean?

Answer 3

> is a symbol that means “greater than

Question 4

What does < mean?

Question 5

For determining values that will give a spontaneous reaction what needs to be done?

Answer 5

Find T where DeltaG = 0 then any T greater than that value will give a spontaneous reaction

Question 6

What is isothermal titration calorimetry? (ITC)

Answer 6

Excellent for studying biomolecular interactions between for example protein-small molecules, protein-protein, target drugs, enzyme exhibitors, and protein-DNA. ITC directly measures the heat released or absorbed during a biomolecular binding event.

Question 7

What is Differential Scanning Calorimetry? DSC?

Answer 7

Used to investigate the stability of biological (and other) systems. Measures the enthalpy of unfolding due to denaturation and the change in heat capacity of denuration.

Question 8

What are heat capacity changes associated with protein unfolding due to?

Answer 8

Changes in the hydration of side chains that were buried in the native state, but become solvent exposed in the denatured state.

Question 9

What does > mean?

Answer 9

> is a symbol that means “greater than

Question 10

What does < mean?

Question 11

For determining values that will give a spontaneous reaction what needs to be done?

Answer 11

Find T where DeltaG = 0 then any T greater than that value will give a spontaneous reaction

Question 12

What is isothermal titration calorimetry? (ITC)

Answer 12

Excellent for studying biomolecular interactions between for example protein-small molecules, protein-protein, target drugs, enzyme exhibitors, and protein-DNA. ITC directly measures the heat released or absorbed during a biomolecular binding event.

Question 13

What is Differential Scanning Calorimetry? DSC?

Answer 13

Used to investigate the stability of biological (and other) systems. Measures the enthalpy of unfolding due to denaturation and the change in heat capacity of denuration.

Question 14

What are heat capacity changes associated with protein unfolding due to?

Answer 14

Changes in the hydration of side chains that were buried in the native state, but become solvent exposed in the denatured state.