Lecture 6 - module 2 Flashcards
What is respiration used for?
Organisms use cellular respiration to extract energy from organic molecules.
What are two categories of how organisms obtain energy?
- Autotrophs
- Heterotrophs
How do autotrophs obtain energy?
They produce their own organic molecules through photosynthesis to break down into energy
How do heterotrophs obtain energy?
They consume organic compounds produced by other organisms to break down into energy.
How do organisms extract energy from organic molecules?
Cellular respiration.
What is the basic formula for cellular respiration?
Sugar (glucose) + 6 Oxygen = 6 carbon dioxide + 6 water + energy
What is free energy?
Large amount of energy that must be released in small steps.
How much free energy can come from one mol of glucose?
-686 kcal/mol or more.
Why does cellular respiration occur in many steps instead of all at once?
Because it increases the amount of energy made and decreases the amount lost.
What do electron carriers do?
Catch energy.
What is an example of an electron carrier?
NAD+ which acquires 2 electrons and a proton to become NADH.
What do cells use ATP for?
To drive reactions.
What are 2 mechanisms for synthesis of ATP?
- Substrate-level phosphorylation
- Oxidative phosphorylation
What is the substrate-level phosphorylation?
It transfers the phosphate group directly to ADP.
What is the oxidative phosphorylation?
Indirect way of making ATP from a proton gradient.
What are 4 steps of cellular respiration?
- Glycolysis
- Pyruvate oxidation
- Citric acid cycle
- Electron transport chain and chemiosmosis
What is the input and output for glycolysis?
1 glucose (6 carbons) = 2 pyruvate (3 carbons)
Where does glycolysis occur?
In the cytoplasm.
What is produced in glycolysis?
Net of 2 ATP and 2 NADH.
What happens with pyruvate after glycolysis?
Oxidation:
1. Aerobic respiration - if there is oxygen
or
2. Fermentation - if there is no oxygen
What is aerobic respiration?
- When pyruvate is oxidized to acetyl coenzyme A (acetyl-CoA) which enters the citric acid cycle.
- oxygen is final electron receptor
- significant amount of ATP produced
What is fermentation?
- When pyruvate is reduced in order to oxidize NADH back to NAD.
- oxygen is not available
- organic molecule is the final electron acceptor
What must happen to NADH?
It must be recycled so the process can continue.
What is pyruvate oxidation?
When pyruvate is in the presence of oxygen, it is oxidized.
Where does pyruvate oxidation occur in prokaryotes and eukaryotes?
In eukaryotes it occurs in the mitochondria and in prokaryotes it occurs in the plasma membrane.
What are the products from the oxidation of one 3-carbon pyruvate?
1 CO2
1 NADH
1 acetyl-COA.
What is the citric acid cycle?
When the acetyl group from pyruvate is oxidized.
Where does the citric acid cycle occur?
The matrix of the mitochondria.
What are the steps of the citric acid cycle?
- Acetyl-CoA + oxaloacetate = citrate
- Citrate rearrangement of decarboxylation
- Regeneration of oxaloacetate
What is the product from one Acetyl-CoA in the citric acid cycle?
2 CO2r
3 NADH
1 FADH2
1 ATP
What is the electron transport chain (ETC)?
A series of membrane-bound electron carriers.
Where is the electron transport chain?
Embedded in the inner mitochondrial membrane.
What does the ETC do?
- Transfers electrons to complexes of ETC and passes them along
- Each complex operates as a protein pump, driving protons to the intermembrane space
What is chemiosmosis?
Accumulation of protons in the intermembrane space drives protons into the matrix via diffusion.
How do protons reenter the matrix?
ATP synthase.
How is ATP synthesis carried out?
By tiny rotary motor driven by protein gradient. Protons travel through the stalk and cause the head to rotate.
What is the theoretical energy yield of respiration?
30-32 ATP
How does anerobic respiration occur without oxygen?
The final electron acceptor is an inorganic molecule other than oxygen. Many prokaryotes use sulfur, nitrate, carbon dioxide.
What are the two oxidation processes that can occur without oxygen?
- Anaerobic respiration
- fermentation
What are 2 types of anaerobic respiration?
- methanogens
- sulfur prokaryotes