Lecture 5b: Ras-Myc Oncogene Addiction

Question 1

Insertional Mutagenesis

Answer 1

Slowly transforming retroviruses activate proto-oncogenes by inserting their genomes adjacent to these cellular genes

Question 2

pBKI0 Mice

Answer 2

Ras expression is tissue-specific because Ras expression is under the control of pBKI0 promoter, expressed in skin basal cells in mice.

Question 3

Ras Activation Pathway

Answer 3

1. Growth factor
2. Interaction with receptor
3. Intracellular signaling
4. Activations of kinases
5. New transcription factors
6. Expressions of various genes

Question 4

Oncogene addiction

Answer 4

Describes the dependency of certain tumor cells on a single activated oncogenic protein or pathway to maintain their malignant properties, despite the likely accumulation of multiple gained loss-of-function mutations that contribute to tumorigenicity