Lecture 5 DA Flashcards
What does the Arrhenius law state?
Reaction rate of simple reactions can be increased by increasing temperature.
What is the equation to Arrhenius law?
How do enzymes reduce the activation energy?
By stabilising the transition state.
How can you increase reaction rates?
More enzyme More substrate (only works if enzyme isnt saturated) Higher temperature Prevent product accumulation
What is a holoenzyme, and apoenzyme?
Apoenzyme is an inacitvated enzyme prior to binding to a cofactor. Is a holoenzyme after binding.
What are cofactors, coenzymes and prosthetic groups?
Cofactors are molecules that increase the rate of reaction or are required for enzyme function. Coenzymes are are organic molecules that bind to enzymes for proper function.Prosthetic groups are cofactors that bind tightly to enzymes.
What is Vmax and Km?
Vmax - maximum reaction rate Km - 50% of Vmax
Is E + S -> ES a first order or second order reaction, and why? What about ES -> P + E?
E + S -> ES is a second order reaction because it depends on two species to go forward. ES -> P + E is a first order reaction because it depends on one species to go forward.
What is k1?
Forward reaction rate of E+S->ES
What is k-1?
Reverse reaction rate of E+S->ES. ie reaction rate of ES->E+S.
What is k2?
Forward reaction rate of ES->P+E.
Which of the following is reversible?
E + S -> ES
ES -> P + E
E + S -> ES is reversible. ES -> P + E is irreversible.
How do you calculate Vmax?
Vmax = k2 x [Eo]
Eo = initial enzyme concentration
What is the Michaelis Menten equation?
