Lecture 11 DA Flashcards
To which carbon does the phosphate group attach to in nucleic acids? Where will the next nucleotide join to, and what group is found here?
5’ carbon.
Next nucleotide joins to the 3’ hydroxyl.
What is found at the 2’ carbon in DNA vs RNA?
DNA - H
RNA - OH
What bases are purines, and how many rings do they have? What about the pyrimidine bases?
Purines
A, G - are double ringed
Pyrimidines
C, U, T - are single ringed
If a base has a sugar attached, what is the naming convention?
-sine suffix is added.
How can genes be inactivated?
Methylation.
What is the link between nucleotides?
Phosphodiester bond.
What wavelength do nucleotides absorb highly at, and why?
At 260nm, due to their ring structure.
How long is an AT bond? What about GC? How many pairs of H bond each?
AT - 11.1A long, 2 H bonds
GC - 10.8A long, 3 H bonds
Which is stronger, AT or GC bonds?
GC
How much space is between each nucleotide?
Bases are spaced out 3.4A apart.
How wide is DNA?
10A
What are the minor and major grooves?
Minor grooves are where the DNA pairs bind, between them are the major grooves.
How long is one turn of the DNA?
36A.
What direction does DNA synthesis occur?
5’ to 3’.
In DNA synthesis/replication, are both strands synthesised continuously?
No, the 3’ to 5’ direction is synthesised in pieces at a time known as okazaki fragments and later ligated by DNA ligase.
Name the three forms of DNA. Which is the most common, and are they left handed or right handed?
A form - right handed, like the B form, but shorter and thicker.
B form - right handed, most common.
Z form - left handed, very unstable.
What is the base pair sequence of Z form DNA?
Alternating purines and pyrimidines.