lecture 5- bacterial diversity Flashcards
High G+C
Actinobacteria
Actinobacteria
Actinomyces
Frankia
Streptomyces
Actinomyces
filamentous, branching, some human pathogens
Frankia
filamentous
Nitrogen fixation
Streptomyces
Forms mycelium produces antibiotics: tetracycline macrolides aminoglycosides amphotericin B
Low G+C
Firmicutes
Firmicutes
Lactobacillus Streptococcus Staphylococcus Bacillus Clostridium
Gram negative
proteobacteria (alpha, beta, gamma, epsilon)
alpha
Rhizobiales: agrobacterium bradyrhizobium nitrobacter rhizobium
nitrogen fixing bacteria
bradyrhizobium
rhizobium
frankia
nitrifying bacteria
nitrobacter
what is nitrogen fixation
Nitrogen –> ammonia
beta
neisseria
characteristic of neisseria
diplococci
gamma
enterobacteriales:
shigella
salmonella
E.coli
epsilon
camplylobacteriales:
campylobacter
helicobacter
characteristic of helicobacter
multiple flagella
what kind of fluorescent oligonucleotides can be used?
DAPI (4,6-diamidino-2-phenylindole) 400 nm
Acridine orange 500 nm
SYBR Green 497 nm
how to phylogenetically classify non-culturable bacteria?
study 16s rRNA gene of the bacteria
FISH
fluorescent in situ hybridisation uses different coloured oligonucleotides to match different 16s rRNA sequences
species richness
the total number of species present in an ecosystem
species abundance
the proportion of each species in an ecosystem
pelagic zone
nutrient poor
prochlorophytes
- related to cyanobacteria
- photosynthetic prokaryote member of the phytoplankton group Picoplankton.
- abundant in nutrient poor tropical waters
- use a unique photosynthetic pigment, divinyl-chlorophyll, to absorb light and acquire energy.
photic zone
200m below surface
pelagibacter ubique
nonphototrophic oligotroph
archaea
found in extreme conditions below the photic zone
most abundant bacteria found in colon
firmicutes
bacteroids
swollen, misshapen and branched rhizobia
leghaemoglobin
enzyme produced by nodules, keeps O2 away from nitrogenase
where does nitrogenase function
in bacteroids but not free living rhizobia
flavonoids
stimulate growth of rhizobia
nod factors
induce root hair curling and trigger plant cell division