Lecture 5 Flashcards
What are some properties of Polyacrylamide gel electrophoresis (PAGE)
- More dense
- longer gels
- vertical
- poly process start and mix pour btwn glass
- cheap
- bad chem, annoying process
Why is it desireable to have denaturing conditions in the PAGE gel?
By including molecules like urea or formamide and keeping the gel hot, it facilitates h bonds btwn it and the base pairs, preventing complimentary base pairing
What are some properties of capillary electrophoresis?
- Thin glass tubes w viscous polymer
- denaturing chemicals + heat
- electrostatic injection of sample
- robot!
What r properties of single capillary 310?
- slow (1 @ a t)
- easy and sensitive af
- worse temp ctrl
What would cause miscalling on an allele on the 310?
The allelic ladder was analyzed at a seperate time than the sample = change in electrophoretic cond would cause the miscall
Know what this acronym means: AMP FLP or AFLP
Amplified fragment length polymorphism
How are tandem repeats classified?
By how many nucleotides the repeat is, ex. tetranucleotide: 4
What are Simple TR?
all identical
What are compound TR
more than one sequence but all the same length
What are complex TR?
include mixture of diff lengths (not in FS)
When do Microvarients occur?
When there is a single repeat thats missing one or more bases
Micro variant alleles are named by
Adding 0.1 for ea base in the short repeat
What was the first commonly used minisatellite? Why?
D1S80
at the shortest range for repeats, 14 bp
making it v discriminating for a single AMP FLP locus
What is a microvariant
an incomplete repeat for a given allele. if the repeat in question is AGAA, then if the allele is 14.2, it means there is 14 AGAA repeats, and an extra AG at the end. (the .2)
