Lecture 19

Question 1

What regions of the control region are sequenced for human id

Answer 1

Hypervariable region 1 or 2

Question 2

for HV1 and HV2, which has larger numbers (as in, where it is in the sequence of mtDNA)

Answer 2

HV1 = higher numbers
HV2 = smaller numbers

Question 3

What can mtDNA be used for

Answer 3

• mass disasters where tissue samples are badly damaged
• ## aka degraded DNA

Question 4

Paternal leakage

Question 5

What does the high mutation rate of mtDNA reflect

Answer 5

The lack of DNA repair mech and chemically reactive environment w free radicals

Question 6

Is mtDNA more stable in living organisms or dead?

Answer 6

Dead.

Question 7

Why is mtDNA a good source for ID in forensics? (3)

Answer 7

• single haploid locus w high copy # & mutation r8
• maternally inherited (not as discriminatory as STR)
• resistant to postmortem degradation

Question 8

What is the common reference sequence (standard) for human mtDNA

Answer 8

CRS Cambridge Reference Sequence or unofficially: Anderson sequence

Question 9

How are results of mtDNA communicated?

Answer 9

How results differ from the standard (CRS)
EX: Table of 3 bases at position #’s if there were only 3 differences

Question 10

What version of the CRS is used in analysis today? original or revised?

Answer 10

original