Lecture 4

Question 1

Optogenetics

Answer 1

Method that uses light to modulate molecular events in a targeted manner in living cells or organisms

Question 2

What are some current tools available for optogenetics

Answer 2

Neuronal excitation and inhibition using light sensitive ion channels which either depolarize or hyperpolarize the neuron

Light sensitive protein domains that change conformation

Question 3

How are optogenetic approaches enabling research in cell biology through literature

Answer 3

can study cell motility
protein localization
Gene expression and differentiation
Protein clustering and sequestration

Question 4

What are some conventional techniques for manipulation of protein and their limitations

Answer 4

CRISPR
Chemical probes
Mutagenesis

these don’t allow for spatial and temporal control
Can’t easily reverse a condition