8

Question 1

What is zymography

Answer 1

No reducing agent is used.
Electrophoretic technique for the detection of hydrolytic enzymes. Enzymes are run in a gelatin, and the enzymes degrade the gelatin. White bands = degradation.
Run in native condition

Question 2

Methods to measure activity of MMPs

Answer 2

Zymography
in situ zymography
Fluorometric assay of MMP-9 activitiy

Question 3

What are some processes MMPs are involved in

Answer 3

Bone repair, wound healing, morphogenesis, cell migration

Question 4

What is a MMP

Answer 4

Proteolytic hydrolase enzyme
Exo = cleaves at the ends
Endo = cleaves at the middle
Need metal catalytic action
Use water to cleave proteins

Question 5

goal of paper

Answer 5

Discover MMP-2’s role in the SR. Discover MMP-2’s role in cardiac injury

Question 6

What is ARP-100

Answer 6

MMP inhibitor

Question 7

What is the role of MMP-2 in SERCA2s degradation

Answer 7

Localized within the SR of cardiac cells and directly contributed to SERCA2a proteolysis during IR injury

Question 8

What methods did they use

Answer 8

Zymography
Western blotting
mass spectrometry

Question 9

possible problem with picking ARP-100

Answer 9

Could have inhibited other MMPs which contributed to proteolysis