Lecture 2 Flashcards

1
Q

Barriers to betacoronavirus spillover

A

Host entry and fusion

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Describe the entry and fusion of SARS-CoV-2

A

Entry: Virus attaches to the human cell through interactions with receptor (ACE 2). Cell-surface proteases then cleave the virus and activate it. Following activation, the virus is endocytosed where it is acted upon by lysosomal protease to release the viral genome into the cell. Binding to ACE2 initiates the entry process

Fusion: furin cleavage site allows SARS-CoV-2 fusion

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What drives coronavirus evolution

A

Recombination
When two strains infect a cell, the viruses can replicate and mix to create a hybrid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Evolution and recombination impact what?

A

ACE2 usage
Can spread ACE2 usage

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

how can we assess spillover potential in silico, in vitro, in vivo

A

In silico

Structural homology
Binding prediction
Epidemiological modeling
Ecological modeling

In vitro

Pseudovirus systems
Chimeric viruses
Cell culture
Organoids

In vivo

Mice
Animal models
Non-human primates

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q
A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly