lecture 32-34 - glycan binding proteins - lectins Flashcards
what do lectins bind? with what affinity?
- tend to bind monosaccarides and oligosaccarides
- N-glycans, O-glycans, glycolipids
- few recognize sulfated GAGs
- low affinity
describe N-glycans
- oligosaccarides
- connected to proteins through N-acetyl glucosamine that is N-linked to an Asn res
describe O-glycans
-oligosaccarides
-connected to proteins through an O-linkage to Ser/Thr
(typically connected through N-acetyl glucosamine, but other sugars have been found as well)
glycolipids
- oligosaccarides
- O-linked to lipid chains through glucose or galactose
are lectins branched or unbranched structures? is this the same or the opposite of GAGs?
- branched
- opp of GAGs
where do lectins typically bind glycans?
- terminal grps (minimal binding epitopes=what they bind)
- di, tri, tetra-saccarides displayed at the ends of glycans
are lectins evolutionarily related?
yes
how are lectins classified into groups?
grouped into evolutionarily related families based on carbohydrate-recognition domains (CRDs)
what do Galectins (fam of lectins) bind?
Beta-galactose containing glycans
explain prototypical galectins
- single CRD
- can associate as homodimers
explain chimera galectins
- single CRD
- CRD has a terminal polypeptide tail (usually rich in pro, gly, tyr)
- tail can help form oligomers
explain tandem galectins
- 2 CRDs
- CRDs connected through polypep-linker
- each CRD can have diff galectin binding properties
what are C-type lectins?
- calcium dependent GBPs
- share primary and secondary homology in their CRDs
- are some exceptions to these
explain the CRDs for c-type lectins
- most have a single CRDs
- most are transmembrane proteins
what can c-type lectins do to affect bidning affinity? what is the effect?
- can cluster together
- increase binding affinity