Lecture 21 - Biotechnology in use Flashcards
Can EPO carry out PTM? What cells is it best produced in?
Yes (glycosylation), Mammalian cells (CHO),
Can insulin carry out PTM? What cells is it best produced in?
No, Bacteria
Can antithrombin carry out PTM? What cells is it best produced in?
Yes (carboxylation), Transgenic animals (Goats via pharming)
How do we design new proteins?
- Identify a need
- Target selection
- Design a protein
- Gene synthesis
- Purification and expression
- Characterization and optimization
- Validation and testing.
How can we provide a permanent treatment?
Use patients as the host for protein production,
Gene therapy for diabetes,
How can we continue to feed a growing population?
Next generation dairy products,
Climate-friendly plants that make dairy proteins.
What is gene therapy?
Using viral vectors to insert a recombinant gene into patient cells.
What are the two types of gene therapy?
Integrating and non-integrating
Is gene therapy permanent?
Yes
How could pancreatic B cells being destroyed be permanently treated using gene therapy?
Reprogramming A cell to act as B cells.
What dairy protein is produced without animals?
Casein milk powder (80% of milk protein)
What are some benefits from using plants instead of cows for dairy?
- lower carbon emissions
- Easier mass production at a lower overall cost
- Help feed a growing world population
What factors would you take into account when choosing a system to produce a recombinant protein?
Cost and resources, Stability, Safety, Yield and production
What are the steps required to make a recombinant protein?
- Identify a need
- Target selection
- Design a protein
- Gene synthesis
- Purification and expression
- Characterization and optimization
- Validation and testing.
