Lecture 2 Flashcards
Studying Cells
Hypothesis
A proposed explanation made on the basis of observations, as a starting point for further investigation.
Controlled Experiment
One that tests experimental and control groups in parallel.
What is tested in an investigation?
Prediction
Scientific Theory
A broad explanation supported by many observations, or experiments.
Theory Vs Hypothesis
A hypothesis is a proposed explanation of observations, and a theory is a an explanation that is supported by scientific experiments.
Prokaryotic cells
Single celled organisms such as bacteria.
Eukaryotic cells
Eukaryotic cells contain a nucleus.
Eukaryotic cell size range
10 - 100 micromillimeters.
Prokaryotic cell size range
1 - 10 micromillimeters.
Types of Light Microscopy
Brightfield, Fluorescent, and Confocal Microscopy.
Light Microscopy resolution limit
0.2 micromillimeters.
Fluorescent Microscopy
Light microscopy in which a specific wavelength of light is used to illuminate the specimen, and a longer wavelength of light is collected to form the image
Brightfield Microscopy
Objects are dark and the field is light; can be used to observe stained and unstained microorganisms.
Confocal Microscopy
A light microscope that uses fluorescent stains and laser to make two- and three-dimensional images
Electron Microscopy
Uses electrons instead of light, the shorter wavelength of electrons gives greater resolution.Cannot view living organisms.
