Lecture 19 - ER, Trafficking, Cotranslational Import Flashcards

1
Q

Functions of Smooth ER (SER) (4)

A
  1. Lipid synthesis
  2. Production steroid
  3. Detoxification
  4. Sequestration
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2
Q

Production steroid hormones happens using what compounds

A

like glucocorticoids, androgens, and estrogens — endocrine cells

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3
Q

what is Detoxification

A

liver cells contain
enzymes that modify foreign compounds

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4
Q

what is Sequestration

A

(storage) of Ca2+ in
muscle cells

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5
Q

Functions of Rough ER (RER) (4)

A
  1. Synthesis of membrane phospholipids.
  2. Glycosylation of proteins
  3. Protein folding — quality control
  4. Protein synthesis, modification, and transport
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6
Q

what is Glycosylation of proteins

A

Addition of carbohydrate chains to specific proteins

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7
Q

what does Protein folding — quality control involve

A

the activity of molecular chaperones, a specific type of protein that assist in the folding process

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8
Q

what is this process called?

  • Proteins targeted to ER.
  • Proteins targeted to other endomembrane compartments (soluble and transmembrane).
  • Proteins targeted to plasma membrane (secreted and transmembrane)
A

Protein synthesis, modification, and transport

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9
Q

In the cytoplasm, ribosomes synthesize what from what?

A

polypeptides from mRNA

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10
Q

All protein translation begins on what

A

free ribosomes (i.e. not associated with the ER)

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11
Q

Translation is completed in 1 of 2 ways:

A

FREE or ER BOUND ribosomes

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12
Q

what proteins and molecules are involved in translation completed on free ribosomes?

A
  • Cytosolic proteins
  • Peripheral membrane proteins
  • Proteins targeted to nucleus, mitochondria, peroxisomes, chloroplasts
    RER and Protein Translation
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13
Q

For translation completed by ribosomes associated with ER membrane, what proteins are involved?

A
  • Secreted proteins
  • Integral membrane proteins
  • Soluble proteins associated with inside (lumen) of endomembrane system (i.e. proteins that function within the ER, Golgi, lysosomes)
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14
Q

How is the site of translation determined?

A

Ribosomes are targeted to the ER membrane by a specific signal
sequence

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15
Q

The signal sequence is Located in

A

the amino-terminus (N-terminus)

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16
Q

The signal sequence Contains several

A

consecutive hydrophobic amino acids

17
Q

The signal sequence directs

A

synthesis to the ER compartment

18
Q

Protein moves through channel into ER for what

A

cotranslational import

19
Q

in Cotranslational Protein Import, After translation of _______ ______, a Signal Recognition Particle (SRP) binds to signal sequence and ______ the translation process.

A

SIGNAL SEQUENCE, STOPS

20
Q

in Cotranslational Protein Import, SRP binds _____ ____ to target the whole translation complex to ____ (SRP + ribosome + mRNA + new polypeptide)

A

SRP receptor, ER

21
Q

in Cotranslational Protein Import, _____ is released and ribosome binds to ________. Once this is done, protein synthesis resumes

A

SRP, translocon

22
Q

in Cotranslational Protein Import, ________ enters the ER (through the translocon) as it is translated. In the end,
the signal peptide is _______ ____ and chaperone folds the protein.

A

Polypeptide, cleaved off

23
Q

Movement of cellular components:
To the plasma membrane via biosynthetic/secretory pathway =

A

exocytosis

24
Q

Movement of cellular components: From the plasma membrane → organelle within cell via endocytic pathway =

A

endocytosis

25
Q

Examples of exocytosis:

A

Organelle → Plasma membrane
Secretion of neurotransmitter from
pre-synaptic neuronal cells

26
Q

Examples of endocytosis:

A

Plasma membrane → Organelle
Uptake of Low Density Lipoprotein (LDL) particle in liver cells