Lecture 19 - ER, Trafficking, Cotranslational Import Flashcards
Functions of Smooth ER (SER) (4)
- Lipid synthesis
- Production steroid
- Detoxification
- Sequestration
Production steroid hormones happens using what compounds
like glucocorticoids, androgens, and estrogens — endocrine cells
what is Detoxification
liver cells contain
enzymes that modify foreign compounds
what is Sequestration
(storage) of Ca2+ in
muscle cells
Functions of Rough ER (RER) (4)
- Synthesis of membrane phospholipids.
- Glycosylation of proteins
- Protein folding — quality control
- Protein synthesis, modification, and transport
what is Glycosylation of proteins
Addition of carbohydrate chains to specific proteins
what does Protein folding — quality control involve
the activity of molecular chaperones, a specific type of protein that assist in the folding process
what is this process called?
- Proteins targeted to ER.
- Proteins targeted to other endomembrane compartments (soluble and transmembrane).
- Proteins targeted to plasma membrane (secreted and transmembrane)
Protein synthesis, modification, and transport
In the cytoplasm, ribosomes synthesize what from what?
polypeptides from mRNA
All protein translation begins on what
free ribosomes (i.e. not associated with the ER)
Translation is completed in 1 of 2 ways:
FREE or ER BOUND ribosomes
what proteins and molecules are involved in translation completed on free ribosomes?
- Cytosolic proteins
- Peripheral membrane proteins
- Proteins targeted to nucleus, mitochondria, peroxisomes, chloroplasts
RER and Protein Translation
For translation completed by ribosomes associated with ER membrane, what proteins are involved?
- Secreted proteins
- Integral membrane proteins
- Soluble proteins associated with inside (lumen) of endomembrane system (i.e. proteins that function within the ER, Golgi, lysosomes)
How is the site of translation determined?
Ribosomes are targeted to the ER membrane by a specific signal
sequence
The signal sequence is Located in
the amino-terminus (N-terminus)