Lecture 17 Flashcards
endosymbiotic
-margulius
-aerobic bacteris is the mitochondria and it still divides by fission
-cyanobacteria is the chloroplast
extra nuclear inheritance
maternal and uniparental
-all children inherit mom mitochondria
-can use donor egg with your nucleus is you have mitochondrial mutation
Barbara mc clintok
-corn genes
-chromoson 9 breaks and spotted kernels happen
-mapped DS to the break
the break is triggered by
AC
-Purple with yellow spots
DS pops out
the purple color is restored/ normal
-offspring will be normal
DS
jumping gene/ transposable
autonomous DS
carries transposoase
-the transposoase is controlled by the transposon
transposoase
before the repressor gene in the sequence
-encoded by AC and binds at IR to move DS
HIV
retrovirus that integreates into DNA like lambda phage
-host TF control the expression of the gene and make the virus
-brings RT with it
-has terminal repeats
gag
capside
pol
rt and integrase
HIV transposase
integrase
retrotransposone
transcribe RNA intermediate to DNA and insert it to target and leave a cop in donor
-RNA mediates the transduction
retrotransposone model
morgan white eye fly
LINES
interspersed sequence that encode transposase and RT
-uses an RNA intermediate
SINE
need LINE enzymes
transposones cause?
alternative splicing, elongation defect and RNAi production
genes with transposones have
an altered percent of CG
germline tranposone
hereditary
somatic transposone
mosaic and not hereditary (corn w spots)
repetitive sequences and retrotransposones can be silenced by?
CPG methylation, histone methylation or miRNA and MECP2
what do transposones look like?
RAG 1 and 2
TBXT
TF that regulates genes and is on in many cancers
ALU SINE at intron 5 TBXT mutation
tailless= het
-skip at exon 6 in the gel (shorter band)
-homo is lethal
