Lecture 17

Question 1

endosymbiotic

Answer 1

-margulius
-aerobic bacteris is the mitochondria and it still divides by fission
-cyanobacteria is the chloroplast

Question 2

extra nuclear inheritance

Answer 2

maternal and uniparental
-all children inherit mom mitochondria
-can use donor egg with your nucleus is you have mitochondrial mutation

Question 3

Barbara mc clintok

Answer 3

-corn genes
-chromoson 9 breaks and spotted kernels happen
-mapped DS to the break

Question 4

the break is triggered by

Answer 4

AC
-Purple with yellow spots

Question 5

DS pops out

Answer 5

the purple color is restored/ normal
-offspring will be normal

Question 6

DS

Answer 6

jumping gene/ transposable

Question 7

autonomous DS

Answer 7

carries transposoase
-the transposoase is controlled by the transposon

Question 8

transposoase

Answer 8

before the repressor gene in the sequence
-encoded by AC and binds at IR to move DS

Question 9

HIV

Answer 9

retrovirus that integreates into DNA like lambda phage
-host TF control the expression of the gene and make the virus
-brings RT with it
-has terminal repeats

Question 10

gag

Answer 10

capside

Question 11

pol

Answer 11

rt and integrase

Question 12

HIV transposase

Answer 12

integrase

Question 13

retrotransposone

Answer 13

transcribe RNA intermediate to DNA and insert it to target and leave a cop in donor
-RNA mediates the transduction

Question 14

retrotransposone model

Answer 14

morgan white eye fly

Question 15

LINES

Answer 15

interspersed sequence that encode transposase and RT
-uses an RNA intermediate

Question 16

SINE

Answer 16

need LINE enzymes

Question 17

transposones cause?

Answer 17

alternative splicing, elongation defect and RNAi production

Question 18

genes with transposones have

Answer 18

an altered percent of CG

Question 19

germline tranposone

Answer 19

hereditary

Question 20

somatic transposone

Answer 20

mosaic and not hereditary (corn w spots)

Question 21

repetitive sequences and retrotransposones can be silenced by?

Answer 21

CPG methylation, histone methylation or miRNA and MECP2

Question 22

what do transposones look like?

Answer 22

RAG 1 and 2

Question 23

TBXT

Answer 23

TF that regulates genes and is on in many cancers

Question 24

ALU SINE at intron 5 TBXT mutation

Answer 24

tailless= het
-skip at exon 6 in the gel (shorter band)
-homo is lethal