[Lec 7] iClicker Questions: Regulation of Prokaryotic Transcription Flashcards
Which of the sites on the diagram below is the most likely location of repressor binding site?
b.
Which of the sites on the diagram below is the most likely location of activator binding site?
a.
You have introduced a mutation into lacI that causes the Lac repressor to lose its ability to bind allolactose. How will this affect expression of the lac operon?
A) The lac operon will always be activated
B) The lac operon will never be activated
C) The lac operon will be unaffected
b.
Scientists sometimes use synthetic molecules in the lab that can act as inducers and/or substrates for β-galactosidase (lacZ).
Consider the molecule IPTG. IPTG mimics allolactose and can bind the Lac repressor. You add IPTG to a culture of E. coli that contains no sugar of any kind. How will lac operon expression be affected?
A) The lac operon will be activated
B) The lac operon won’t be activated
C) The lac operon will be unaffected
a.
Scientists sometimes use synthetic molecules in the lab that can act as inducers and/or substrates for β-galactosidase (lacZ).
Consider the molecule IPTG. IPTG mimics allolactose and can bind the Lac repressor. You add IPTG to a culture of E. coli that contains an abundant amount of glucose. How will lac operon expression be affected?
A) The lac operon will be activated
B) The lac operon wont’ be activated
C) The lac operon will be unaffected
b.
You have introduced a mutation into the operator region upstream of the lac operon. You begin growing the bacteria in media that contains no sugar. How will lac operon expression be affected?
A) The lac operon will be activated
B) The lac operon wont’ be activated
C) The lac operon will be unaffected
a.
You have introduced a mutation into CAP that causes it to lose its ability to bind cAMP. How will this affect expression of the lac operon?
A) The lac operon will be activated
B) The lac operon won’t be activated
C) The lac operon will be unaffected
b.
Considering the diagram below, select the correct environmental conditions.
A) High glucose, high lactose
B) High glucose, low lactose
C) Low glucose, high lactose
D) Low glucose, low lactose
d.
Considering the diagram below, select the correct environmental conditions.
A) High glucose, high lactose
B) High glucose, low lactose
C) Low glucose, high lactose
D) Low glucose, low lactose
b.
Considering the diagram below, select the correct environmental conditions.
A) High glucose, high lactose
B) High glucose, low lactose
C) Low glucose, high lactose
D) Low glucose, low lactose
a.
Considering the diagram below, select the correct environmental conditions.
A) High glucose, high lactose
B) High glucose, low lactose
C) Low glucose, high lactose
D) Low glucose, low lactose
c.
Which of the following condi@ons gives results in fully ac@vated transcrip@on for AraBAD operon?
A) High glucose, high arabinose
B) Low glucose, low arabinose
C) High glucose, low arabinose
D) Low glucose, high arabinose
d.
