[Lec 2] iClicker Questions: Structure of RNA

Question 1

A) X is the cytoplasm and Y in the mitochondria

B) X is the cytoplasm and Y is the nucleus

C) X is the nucleus and Y is the cytoplasm

D) X is the nucleus and Y is the mitochondria

Answer 1

c.

Question 2

The experiments used to determine the “central dogma” were done in eukaryotic cells. If these experiments were done in prokaryotic cells would you expect the same results?

A) Yes

B) No

Answer 2

b.

Question 3

You begin your studies in a new lab on a novel pool of RNA. Your boss has asked you to assign it to a known categorization. This pool consists of RNA of uniform length. You can detect amino acids but not proteins. Your RNA is:

A) mRNA
B) tRNA
C) rRNA
D) Either tRNA or rRNA

Answer 3

b.

Question 4

Which of the following correctly describes the shapes of the major and minor grooves in double-helical RNA?

A. The major groove is narrow and deep, the minor groove is wide and shallow

B. The major groove is wide and shallow, the minor groove is narrow and deep

C. The major groove is wide and shallow, the minor groove is narrow and shallow

D. Both the major and minor grooves are wide and shallow

Answer 4

a.

Question 5

Suppose a mixture of RNA and DNA is treated with alkali. What will be the result?

A) Both the RNA and DNA will be denatured.

B) Both the RNA and DNA will be degraded.

C) The RNA will be degraded and the DNA will be denatured.

D) The RNA will be degraded and the DNA will remain the same.

Answer 5

c.

Question 6

Would you expect Chargaff’s rules (%A=%T, %G=%C) to hold for RNA?

A) Yes

B) No

Answer 6

b.

Question 7

What you are looking at is a western blot, which can detect protein levels. The darker the band the more protein which is in the sample. Both fHbp and RecA are proteins produced by a pathogenic bacteria. Which one (if any) you anticipate is regulated by a thermosensor?

A) fHbp

B) RecA

C) Both proteins

D) Neither protein

Answer 7

a.

Question 8

Again, you are looking are protein levels, this 4me on a graph where the more “Miller Units” the more protein is being made. Each line is a mutation made on the mRNA of this protein. Which mutation abolishes the thermoregulation of this protein?

A) 1

B) 2

C) 3

D) 4

E) 5

Answer 8

e.

Question 9

A new pathogenic bacteria, which normally grows at 37oC, has been discovered. Do you predict that it will regulate virulence factors with thermoswitches?

A) Yes, all pathogenic bacteria use these

B) Yes, since it grows at 37oC this bacteria can use thermoswitches

C) No, because the temperature it grows at is the same as the human body

D) No, because it grows at 37oC normally

Answer 9

c.

Question 10

Which one of the following reactions is NOT catalyzed by a ribozyme?

A) formation of phosphodiester bonds during transcription (i.e, synthesis of complementary RNA from DNA)

B) cleavage of precursor RNAs into mature tRNAs

C) removal of introns during trans-esterification reactions involved in certain mRNA splicing

D) formation of peptide bonds during protein synthesis

Answer 10

a.