[Lec 6] iClicker Questions: Transcription II

Question 1

Which of the following hypothesis is true?

A) Eukaryotic promoters are more complicated because the polymerase is more complicated

B) Eukaryotic promoters are less complicated because the polymerase is more complicated

C) Eukaryotic promoters are less complicated because the polymerase is less complicated

D) Eukaryotic promoters are more complicated because the polymerase is less complicated

Answer 1

a.

Question 2

The preinitiation complex will form if the TATA box is not present?

A) True

B) False

Answer 2

a.

Question 3

Why does TBP show such a strong preference to T:A regions?

A) T:A base pairs are smaller than G:C base pairs

B) T:A base pairs bind phenylalanine better

C) T:A base pairs are easier to distort

D) T:A base pairs bind the beta-sheet better

Answer 3

c.

Question 4

Which is the correct stepwise assembly of the RNA Pol II preinitiation complex?

Answer 4

b.

Question 5

During mutational studies you discover two cell lines with defects in mRNA transcription. Cell line A has no preinitation complex and cell line B the polymerase never leaves the promoter. Which deletion(s) are in each cell line?

A) Cell line A has TFIIA deleted and cell line B has TFIIH deleted

B) Cell line A has TFIIA deleted and cell line B has TFIIH or TFIIE deleted

C) Cell line A has TFIID deleted and cell line B has TFIIH or TFIIE deleted

D) Cell line A has TFIID deleted and cell line B has TFIIF deleted

Answer 5

c.

Question 6

What steps in the transcription cycle are stimulated by phosphorylation of the CTD of RNA Pol II?

A) Promoter escape

B) Recruitment of RNA processing factors

C) Efficient elongation

D) All of the above

E) None of the above

Answer 6

d.

Question 7

Which of the following amino acids, that if all of them were removed from the CTD tail of Pol II would abolish elongation

A) Tyrosine

B) Serine

C) Lysine

D) Proline

Answer 7

b.

Question 8

The Poly-A tail is encoded in the DNA of Pol II transcripts.

A) True

B) False

Answer 8

b.

Question 9

What purposes do capping and the poly-A tail serve for mRNAs?

A) The 5’ cap protects RNA from being degraded

B) The poly-A tail helps in stability

C) The 5’ cap helps RNA being recognized by RNase

D) Both A and B

E) Both B and C

Answer 9

d.

Question 10

Based on the mechanism of action of alpha amanitin (and the graph below), which of the follow statements is true;

A) At high concentrations only rRNA is made

B) At high concentrations only tRNA is made

C) At high concentrations only mRNA is made

D) At low concentrations both mRNA and rRNA are made

Answer 10

a.