[Lec 5] iClicker Questions: Transcription I Flashcards
The DNA replication enzyme makes 1 mistake in every 10,000,000 base pairs! RNA polymerase makes mistakes
A) Less frequently
B) At the same frequency
C) More frequently
D) Never make a mistake under any circumstance
c
If you take two genetically identical bacteria, and grow them in two different medias (nutrients to support their growth), they will;
A) Transcribe the same genes because they are genetically identical
B) Transcribe the same genes because they are growing in either media
C) Transcribe different genes because they are in different media
D) Transcribe different genes because the media changed their DNA
d.
Give then fact you know bacterial RNA Pol and Pol II in eukaryotic cells are similar which of the following statements is most correct;
A) All regions of the enzyme will be highly similar
B) The outside of the enzyme will be highly similar
C) The catalytic site will be highly similar
c.
Genes can be coded on either strand of DNA. Consider these two genes with coding regions designated by arrows:
For Gene A, which strand will be the template strand?
A) The top strand
B) The bottom strand
C) No idea
b.
Genes can be coded on either strand of DNA. Consider these two genes with coding regions designated by arrows:
For Gene B, which strand will be the coding strand?
A) The top strand
B) The bottom strand
C) No idea
b.
If you are designing a promoter and want a lot of mRNA made, which elements would you include?
A) -10
B) -35
C) Up-element
D) Only A and B
E) A,B and C
e.
Given the following 5 sequences, what is the consensus sequence?
A) AGAATA
B) AGAACT
C) AAAATA
D) AAAGTA
a.
Promoter mutations can affect transcription initiation. Which one of these mutations would make the best promoter?
A) Wild Type
B) Mutant 1
C) Mutant 2
D) Mutant 3
d.
You are testing out an RNA Polymerase you have isolated, however you only get short aborted transcripts. Which option below best fits this scenario;
A) The complex contains no sigma factor
B) The sigma factor can not disassociate from the core of the polymerase
C) The RNA polymerase cannot form a functional holoenzyme
D) The β and α subunits are not recognizing the promoter
b.
What is the correct order of the steps of transcription initiation?
- Unwinding DNA (open complex)
- Release sigma, promoter escape
- Binding RNA polymerase holoenzyme to promoter (closed complex)
- Abortive initiation
A. 1,3,4,2
B. 3,1,2,4
C. 1,3,2,4
D. 3,1,4,2
d.
In which direction does Rho move?
A. 5’ to 3’
B. 3’ to 5’
C. Both
D. Neither
a.
