LEC 5 Flashcards
PROTEINS AND NUCLEIC ACIDS
Protein
long strings made up of amino acids
Amino acids
single units
Amino acid structure
- amino group (-NH3) on one end
- carboxyl (-COOH) on the other end
How many amino acids does our body make?
11 (rest of them (9) needed by diet)
How many amino acids are needed by the human body?
20
Polypeptide
Groups of 3 to 100 amino acids joined together via dehydration synthesis (b/w EACH amino acid)
Polypeptide longer than 100 amino acids?
called a protein
Function of proteins in humans (4 points)
- Structural support
- Muscle contraction
- Cell membrane part
- Enzymes
Function of proteins in the cell membrane (2 points)
- Transmission of info b/w cells
- transportation of materials in and out
Function of proteins in enzymes
regulate rates of biochem reactions
Levels of protein structure (4)
Primary
Secondary
Tertiary
Quaternary
Primary structure (3 points)
- Specific order of amino acids
- Held together by covalent bonds (PEPTIDE BONDS)
- 3 letter code for each amino acid
Secondary structure
How chain is orientated in space
Types of secondary structure (2)
- Alpha Helix
- Beta - sheet
Alpha Helix (2 points)
right-handed spiral
H+ bond b/w amino acids stabilize the structure at regular intervals
Beta Sheet
H+ bonds joins two primary sequences side by side
Tertiary structure
How the protein twists and folds to form a 3D shape
Tertiary structure conditions (3)
- Amino acids involved
- Disulfide bonds
- Polarity of amino acids
Quaternary structure conditions (3)
- More than one polypeptide to make up protein
- How many protein chains involved
- How are they associated with each other
Enzyme
protein that acts like a biological catalyst
Catalysts
speeds up the rate of chem reactions w/o being changed or destroyed
How is an enzyme used? (4 steps)
- Reactants approach enzyme
- Reactants bind to enzyme
- Enzyme changes shape
- Product is released
Nucleic Acids
molecules that store genetic info
DNA (what sugar?)
nucleic acid made with deoxyribose
RNA (what sugar?)
nucleic acid made with ribose
DNA purpose
has instructions for producing RNA
RNA purpose
has instructions for producing proteins
Nucleotides def
smaller subunits that make up RNA and DNA
Nucleotides structure (3)
- Five-carbon sugar
- One or more phosphate group
- Base (single or double-ringed structure containing nitrogen)
Nucleotides in DNA
adenine
thymine
cytosine
guanine
Nucleotides in RNA
adenine
uracil
cytosine
guanine
DNA Structure (4)
- deoxyribose
- base pairs (A+T and C+G)
- phosphate groups
- double stranded
DNA Bonds b/w phosphates and sugars
Covalent bonds
DNA Bonds b/w the two strands
H+ bonds
RNA Structure (3)
- ribose
- base pairs (A+U and C+G)
- shorter than DNA
ATP (3)
universal energy source
same as adenine + 2 extra phosphate groups attached
lots of stored energy b/w the bonds of the 3 phosphate groups
How is energy released from ATP?
Break the bond b/w the last two phosphates using HYDROLYSIS and release energy