Lec 05- Vision Flashcards
What is refraction?
bending of light waves at an angulated surface of a transparent material
What happens to light after striking a perpendicular surface?
continues on through WITHOUT bending
What 2 things does the degree of refraction depend on?
- Ratio of 2 refractive indices of the 2 transparent media
- Degree of angulation between the interface and the entering wave front of the light waves
What is refractive index?
ratio of the velocity of light in air/velocity of light in substance
What is the RI of air?
1
What is refractive power?
how much a lens bends light waves
diopters
How much is 1 diopter?
1 meter/focal length of lens
What is the distance from the center of the lens to the focal point?
focal length
Which lenses don’t have a focal point because they are diverging?
concave lenses
The object as projected on the retina will be _________ and _________
- reversed
- inverted
What does the iris do?
controls the amount of light entering the eye from darkness to light conditions
What does depth of focus of the lens do as the pupillary diameter decreases?
increases
What is Emmetropia?
- Normal eye (depth of focus)
- All distant objects seen clearly
- When ciliary muscle relaxed= parallel light rays from distant objects are in sharp focus on the retina
What is Hyperopia?
- Farsightedness
- Eyeball = too short
- Lens system = too weak (occasionally)
- All distant object seen clearly
What is Myopia?
- Nearsightedness
- When ciliary muscle relaxed= light rays from distant objects are focused in front of the retina
- Eyeball = too long
How is Myopia fixed?
Diverging lens
How is Hyperopia fixed?
Converging lens
Light from a distant point source will normally create a spot with a diameter of _________ and a center that is brighter than the periphery.
11 um
What is the average diameter of a cone in the retina?
1.5 um
What is the max visual acuity for two-point sources of light?
1.5 - 2mm
Which humor is formed by the ciliary processes?
aqueous humor
What is the ciliary apparatus consist of?
2 opposing layers of epithelial cells with a space between them
What ions are actively transported into the space of the ciliary apparatus?
Na+
Cl-
HCO3
What do the ions do in the ciliary apparatus?
- draw water (osmosis) into the space
- aqueous solution then passed into anterior chamber
Fluid flows from the anterior chamber into _______________ and then into aqueous veins in the sclera
Canal of Schlemm
What is normal intraocular pressure?
15 mmHg
What is glaucoma?
-
What is the photosensitive pigment in cones?
rhodopsin
What kind of proteins are photosensitive pigments?
transmembrane conjugated proteins
What are the 4 major functional segments of rods and cones?
- Outer segment
- Inner segment
- Nucleus
- Synaptic body
What major functional segment of rods and cones is the site of light-sensitive photo chemicals?
outer segment
What major functional segment of rods and cones connect with horizontal and bipolar cells?
synaptic body
What major functional segment of rods and cones contains organelles (especially mito)?
inner segment
What is rhodopsin?
Scotopsin + 11-cis retinal
Rhodopsin + Light»_space;
Scotopsin + All-trans retinal
What is 11-cis converted to in the Rhodopsin-Retinal visual cycle?
All-trans retinal
Dissociates from rhodopsin
What is Metarhodopsin II?
- intermediate
- excites electrical changes in the rods
What is required to convert All-trans retinal to 11-cis retinal?
- isomerase
- metabolic energy
What are 2 alternate pathways besides the Rhodopsin-Retinal visual cycle?
- All-trans RETINAL <> All-trans RETINOL (vitaminA)
- All-trans RETINOL <> 11-cis RETINOL <> 11-cis RETINAL
What does the excitation of rods cause?
increased negativity of the intrarod membrane potential
hyper polarization of rod membrane
What does the decomposition of rhodopsin cause?
decreased rod membrane conductance for Na+ ions in outer segment
(hyper polarization of rod membrane)
Which segment continually pumps Na+ from inside the rod to outside the rod and K+ in the opposite direction?
Inner segment
What happens when the outer segment is in the dark?
- Membrane is leaky to Na+
- Na+ leak into cell, neutralize the inside negativity
**Reduced electronegativity inside the membrane
(-40mV instead of -70 to -80mV)
What happens when the outer segment is in the light?
1- Rhodopsin exposed to light = decomposes
2- Retinal portion of rhodopsin complex activated
3- Transducin (G-protein) stimulated
4- cGMP phosphodiesterase activated
5- cGMP»_space; 5’GMP (reduction in cGMP)
6- Na+ channels close
7- Photoreceptor = hyperpolarized
What are the protein portions of the photo chemicals of cones?
opsins
**instead of scotopsin
What are the same in rods and cones?
Retinal portions
What colors are cones sensitive to?
- blue
- green
- red
What are the 5 layers of the retina?
- photoreceptors
- horizontal cells
- bipolar cells
- amacrine cells
- ganglion cells
- Light
Axons going to the brain
ganglion cells
What do photoreceptors do?
-transmit signals to outer plexiform layer