LC Instrumentation

Question 1

two tests that Gabe taught me for leaks inside the instrument

whats interesting about the test? physically

Answer 1

Leak and pressure test

leak test plug on the metal that goes to the column

for the pressure test you plug the hole down

IPA, follow the instructions

Question 2

how does the flow flow including the AIV?

Answer 2

degasser, GPV, AIV, AOV

active inlet valve

Question 3

how does the GPV test look

Answer 3

like steps

Question 4

What is another name for the Gameboy?

Answer 4

instant pilot

Question 5

What is Justin’s favorite expression?

Answer 5

snug is great

Question 6

If your pressure is over 5 bar with your purse valve open what should you do?

Answer 6

Change the frit

Question 7

What part of the LC instrument do you use to make the fittings ?

Answer 7

Discuss the importance of creating fittings

Using specifically designated components to avoid dead volume mixing.

Question 8

Before starting a run besides having right column what else should you check?

Answer 8

Make sure you have enough mobile phase and there is a watch vial?

Question 9

Dont forget there is a column flushing SOP.

Answer 9

9971

Question 10

How to prevent bubbles in 50/50 methanol water Mp?

Answer 10

Stir for 30 minutes helps degass

Question 11

What is the thing call that turns to let the sample into stream?

Answer 11

Rotor seal

Question 12

What is the sample syringe on (Agilent) called?

Answer 12

Metering piston

Question 13

How much does the AIVC cost?

Answer 13

Active inlet valve cartridge 200

Question 14

The other side of the LC details 6 ml

Answer 14

Longer column bigger retention times

Question 15

don’t forget LC 100

Answer 15

wash vials

Question 16

Before doing any test injection in empower always do what before anything else

Answer 16

Always hit set up first gabe Shane laughter