Labs Flashcards
Compare the resolution of phase-contrast and light microscopes!
They are the same
What are the advantages of the phase-contrast microscopy over the light microscopy?
Unstained and living objects can be studied
What does the crossed nicol position mean?
The planes of oscillation determined by the polarizer and the analyzer are perpendicular to each other
What kind of materials can be studies in a polarizing microscope at crossed nicol position?
Anisotropic structures (birefringent structures)
Name two anistropic structures!
Keratin in hair, elastic fibers of the aorta
Name two nucleic acid staining histochemical techniques!
Feulgen-reaction, methylgreen staining
Name two materials which can be visualized with PAS-reaction!
Glycogen, glycolipids
Name a dye suitable for stainfree lipids!
Sudan B
What kind of materials can be stained with Sudan B in the adrenal cortex?
Steroids
What reaction stains specifically the lysosomes in the cell?
Acid phosphate reaction?
