Laboratory: WBC and RBC Counting Flashcards
Cell count is higher in adults compared to newborns.
WBC Counting
RBC Counting
Both
Neither
Neither
Inverted “L” rule is applied in counting
WBC Counting
RBC Counting
Both
Neither
Both
There are 2 basic methods in counting: the manual method and using Coulter counter analyzers.
WBC Counting
RBC Counting
Both
Neither
Both
Diluting fluid is a weak acid solution.
WBC Counting
RBC Counting
Both
Neither
WBC Counting
Sample can be both capillary blood and venous blood
WBC Counting
RBC Counting
Both
Neither
Both
Depth factor = 10
WBC Counting
RBC Counting
Both
Neither
Both
HPO is used in counting
WBC Counting
RBC Counting
Both
Neither
Both
Cell count is corrected in the presence of NRBC
WBC Counting
RBC Counting
Both
Neither
WBC Counting
Increased following strenuous physical activity.
WBC Counting
RBC Counting
Both
Neither
Both
Cells are counted in each 5 small square in the upper-left large corner square.
WBC Counting
RBC Counting
Both
Neither
Neither
4 drops of mixture is discarded after mixing.
WBC Counting
RBC Counting
Both
Neither
Both
Area counted factor = 25/5
WBC Counting
RBC Counting
Both
Neither
RBC Counting
Whole blood is aspirated up to 0.5 mark
WBC Counting
RBC Counting
Both
Neither
Both
Source of error: Improper mixing
WBC Counting
RBC Counting
Both
Neither
Both
Cells are counted in 4 large corner squares
WBC Counting
RBC Counting
Both
Neither
WBC Counting
Diluting fluid: Formol citrate
WBC Counting
RBC Counting
Both
Neither
RBC Counting
Diluting fluid is aspirated up to 101 mark
WBC Counting
RBC Counting
Both
Neither
RBC Counting
In healthy individual, WBC falls in the range of _
4.0-11.0 x 10^9/L
WBC above 11.0 x 10^9 L is termed _
Leukocytosis
White count below normal is known as _
Leukopenia
WBC count is slightly higher in the afternoon than in the
morning. True or False?
True
WBC may drop below normal values in parasitic infection, radiation and certain drug therapy. True or False?
False
Rationale: WBC may drop in viral diseases, radiation and drug therapy
Components of Turk’s diluting fluid
Gentian violet
Glacial acetic acid
Water
2% acetic and 1% HCl along with Turks’s diluting fluid is used as WBC diluting fluid. True or False?
True
Kimwipes is a brand of hematocytometer?
False
Rationale: Brand of cloth/wipes
Principle of WBC Count: Whole blood is mixed with a weak acid solution to dilute the blood and hemolyze the WBCs
False
Rationale: RBCs should be the one being hemolyzed
How many drops should be discarded before usage?
4 drops
Reported unit in WBC count
cells/uL
In leukopenia, blood should be drawn up until the _ mark?
1
In leukocytosis, blood should be drawn up until the _ mark?
0.5
Normal RBC range for adult males
4.2-6.0 x 10^12/L
Normal RBC range for adult females
3.6-5.6 x 10^12/L
Normal RBC range for newborn
5.0-6.5 x 10^12/L
Strenuous physical activity tends to _ the red count
Increase
_ red cell count is found in polycythemia vera and secondary polycythemia
Increased
There are two basic methods used for counting red blood cells
The manual method
Electronic cell counter
These solutions are examples of what fluid?
NSS
3.8% sodium citrate
Dacies/Formol citrate
Hayem’s
Toisson’s
Bethell’s
Gower’s
Red Count Diluting Fluid
To facilitate counting and promote lysis of the red blood cells, whole blood is diluted with an isotonic diluting fluid. True or False?
False
Rationale: Isotonic diluting fluid should prevent lysis of RBC
In polycythemia/erythemia, blood should be drawn up until the _ mark?
0.3
In anemia, blood should be draw up until the _ mark?
1
