Laboratory: Reticulocyte Count and Differential Cell Count Flashcards
In infectious mononucleosis, what cell type may show an absolute increased in the blood?
Neutrophil
Lymphocyte
Monocyte
Eosinophil
Lymphocyte
What is the troubleshooting done in reticulocyte counting if the patient has an unusual high hematocrit?
Smaller amount of blood is added
Larger amount of blood is added
Smaller amount of blood is added
Manual differential count is performed to determine the absolute number of each type of white cell present in blood. True or False?
False
Incubation requirement for reticulocyte counting
-4 C, 15 minutes
22 C, 15 minutes
37 C, 20 minutes
60 C, 20 minutes
37 C, 20 minutes
In Miller Disk, the small area is used for counting
Reticulocytes
Mature RBC
Both
Neither
Both
Reticulocyte contains remnant of _ which are present in large amount in the cytoplasm in the precursors which they were derived
RNA and DNA
DNA and Protein
RNA and Ribosome
DNA and Ribosome
RNA and Ribosome
All are supravital stains, except:
New methylene blue
Brilliant cresyl blue
Wright’s
Pure azure B
Wright’s
Corrected reticulocyte count is used if the patient has
Polycythemia
Abnormally low hematocrit
Leukocytosis
Presence of NRBC
Abnormally low hematocrit
The most absolute and most preferred method/unit of reporting differential count
cells/L
What is the purpose of reticulocyte count?
To help in monitoring anemic patients
To determine the state of erythropoietic activity
To diagnose infection or inflammation
To assess bone marrow activity
To help in monitoring anemic patients
To determine the state of erythropoietic activity
To assess bone marrow activity
White cells are counted in consecutive fields from tail towards the head of the smear
Battlement Method
Cross-sectional Method
Longitudinal Method
Longitudinal Method
What is the distribution of WBC during infection?
Shift to the left
Shift to the right
Shift to the top
Shift to the bottom
Shift to the left
In healthy adults, the predominant white cell is the neutrophil. True or False?
True
An important diagnostic tool which reflects the effectiveness of the bone marrow in producing red cells
Differential cell count
RBC Count
WBC Count
Reticulocyte Count
Reticulocyte Count
What objective is used in differential counting?
100x
All conditions have high reticulocyte count, except:
IDA
Blood loss
Aplastic anemia
Hemolytic anemia
Aplastic anemia
If there are 20 retics in 1000 red cells, what is the % retics?
2.0
Coulter counter report the differential cell count in percent and also as an absolute count. True or False?
True
These conditions indicate increase in _ or known as ?
Appendicitis
Myelogenous leukemia
Bacterial infections
Neutrophils
Neutrophilia
These conditions indicate increase in _ or known as ?
Allergies and allergenic reactions
Scarlet fever
Parasitic infections
Eosinophilic leukemia
Eosinophils
Eosinophilia
These conditions indicate increase in _ or known as ?
Viral infections
Whooping cough
Infectious mononucleosis
Lymphocytic leukemia
Lymphocytes
Lymphocytosis
These conditions indicate increase in _ or known as ?
Brucellosis
Tuberculosis
Monocytic leukemia
Subacute bacterial endocarditis
Typhoid
Rickettsial infections
Collagen disease
Hodgkin’s disease
Gaucher disease
Monocytes
Monocytosis
The white cells are counted in consecutive fields as the blood film is moved from side to side
Cross-sectional or Crenellation Method
Ideal method if the smear is thin enough so that the white cells may be identified all the way to the beginning (head) of the smear
Longitudinal Method
Uses a pattern of consecutive fields beginning
near the tail on a horizontal edge
Battlement Method
Automated differential counters report the cells in percent and also as the
number of each cell type/ L of blood (absolute count). True or False?
True
When the differential shows an abnormal distribution of cell types, a _ may be performed
200-cell differential
The results from 200-cell differential are then averaged (divided by 2) and a notation must be made that
200 white cells were counted. True or False?
True
If the differential count shows the presence of immature granulocytes, this is termed as
Shift to the left
Refers to an increased number of hyper segmented
neutrophils
Shift to the right
As the number of total cells counted increases, the amount of variability will _
Decreases
If NRBCs are seen enumerate them separately and reported as _
NRBC/ 100 WBC
Reticulocyte is found in
_ & _
Bone marrow
Peripheral blood
Immature RBC that remains 24 hours in the circulation before they transform into mature RBC
Reticulocyte
Retics contain remnant of _ and _ which were present in large amount in the cytoplasm
Ribosome and RNA
An important diagnostic tool which reflects the effectiveness of the bone marrow in producing red blood cells
Retics Count
The normal value for reticulocyte count
0.5-1.5 per 100 RBCs
0.5% to 1.5%
25-75 x 10^9/L
Reticulocyte count help in monitoring anemic patients
under treatments; determine the state of increased erythropoietic activity; assess bone marrow activity. True or False?
True
Corrected Reticulocyte count is calculated based on a normal hematocrit of
45%
Corrected Reticulocyte Count formula
Patient hematocrit/normal hematocrit times Retics count
The peripheral blood sample is stained with __
Supravital stain
Supravital stains are __ that have the ability to react with
_ and _ of reticulocytes while it still alive
Basic dyes
Ribosomes and nucleic acids
The nucleic acid-dye reaction forms a __ precipitate of granules and filaments
Blue
Examples of supravital stains
Pure azure B
Brilliant cresyl blue
New methylene blue
Total number of red cells is equal to
Reticulocytes and RBC in 10 fields
Retics count normal range in adults
0.5% - 1.5%
Retics count normal range in neonates
2.5% - 6.0%
Formula used for Retics counts in Miller Disk
A/B(9) x 100
___ and use of _ as anti-coagulant can cause
reticulocyte to show pale staining
High Blood sugar
Heparin
An automated procedure for counting retics using flow
cytometry with fluorescent dyes produces slower yet precise and accurate results. True or False?
False
Rationale: Automated procedure for Retics counting produces rapid, precise and accurate results
New Methylene Blue can stain inclusions such as
Holly-Jowell Bodies
Heinz Bodies
Pappenheimer Bodies
Hemoglobin H
These conditions indicates _ retics count?
Effective erythropoiesis
Anemic patient under treatment
Bone marrow assessment after bleeding
Increased
These conditions indicates _ retics count?
Ineffective erythropoiesis
Hypoproliferative condition
Decreased
