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Hematology 1 > Laboratory: Reticulocyte Count and Differential Cell Count > Flashcards

Laboratory: Reticulocyte Count and Differential Cell Count Flashcards

1
Q

In infectious mononucleosis, what cell type may show an absolute increased in the blood?

Neutrophil

Lymphocyte

Monocyte

Eosinophil

A

Lymphocyte

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2
Q

What is the troubleshooting done in reticulocyte counting if the patient has an unusual high hematocrit?

Smaller amount of blood is added

Larger amount of blood is added

A

Smaller amount of blood is added

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3
Q

Manual differential count is performed to determine the absolute number of each type of white cell present in blood. True or False?

A

False

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4
Q

Incubation requirement for reticulocyte counting

-4 C, 15 minutes

22 C, 15 minutes

37 C, 20 minutes

60 C, 20 minutes

A

37 C, 20 minutes

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5
Q

In Miller Disk, the small area is used for counting

Reticulocytes

Mature RBC

Both

Neither

A

Both

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6
Q

Reticulocyte contains remnant of _ which are present in large amount in the cytoplasm in the precursors which they were derived

RNA and DNA

DNA and Protein

RNA and Ribosome

DNA and Ribosome

A

RNA and Ribosome

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7
Q

All are supravital stains, except:

New methylene blue

Brilliant cresyl blue

Wright’s

Pure azure B

A

Wright’s

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8
Q

Corrected reticulocyte count is used if the patient has

Polycythemia

Abnormally low hematocrit

Leukocytosis

Presence of NRBC

A

Abnormally low hematocrit

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9
Q

The most absolute and most preferred method/unit of reporting differential count

A

cells/L

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10
Q

What is the purpose of reticulocyte count?

To help in monitoring anemic patients

To determine the state of erythropoietic activity

To diagnose infection or inflammation

To assess bone marrow activity

A

To help in monitoring anemic patients

To determine the state of erythropoietic activity

To assess bone marrow activity

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11
Q

White cells are counted in consecutive fields from tail towards the head of the smear

Battlement Method

Cross-sectional Method

Longitudinal Method

A

Longitudinal Method

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12
Q

What is the distribution of WBC during infection?

Shift to the left

Shift to the right

Shift to the top

Shift to the bottom

A

Shift to the left

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13
Q

In healthy adults, the predominant white cell is the neutrophil. True or False?

A

True

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14
Q

An important diagnostic tool which reflects the effectiveness of the bone marrow in producing red cells

Differential cell count

RBC Count

WBC Count

Reticulocyte Count

A

Reticulocyte Count

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15
Q

What objective is used in differential counting?

A

100x

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16
Q

All conditions have high reticulocyte count, except:

IDA

Blood loss

Aplastic anemia

Hemolytic anemia

A

Aplastic anemia

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17
Q

If there are 20 retics in 1000 red cells, what is the % retics?

A

2.0

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18
Q

Coulter counter report the differential cell count in percent and also as an absolute count. True or False?

A

True

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19
Q

These conditions indicate increase in _ or known as ?

Appendicitis
Myelogenous leukemia
Bacterial infections

A

Neutrophils
Neutrophilia

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20
Q

These conditions indicate increase in _ or known as ?

Allergies and allergenic reactions
Scarlet fever
Parasitic infections
Eosinophilic leukemia

A

Eosinophils
Eosinophilia

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21
Q

These conditions indicate increase in _ or known as ?

Viral infections
Whooping cough
Infectious mononucleosis
Lymphocytic leukemia

A

Lymphocytes
Lymphocytosis

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22
Q

These conditions indicate increase in _ or known as ?

Brucellosis
Tuberculosis
Monocytic leukemia
Subacute bacterial endocarditis
Typhoid
Rickettsial infections
Collagen disease
Hodgkin’s disease
Gaucher disease

A

Monocytes
Monocytosis

23
Q

The white cells are counted in consecutive fields as the blood film is moved from side to side

A

Cross-sectional or Crenellation Method

24
Q

Ideal method if the smear is thin enough so that the white cells may be identified all the way to the beginning (head) of the smear

A

Longitudinal Method

25
Q

Uses a pattern of consecutive fields beginning
near the tail on a horizontal edge

A

Battlement Method

26
Q

Automated differential counters report the cells in percent and also as the
number of each cell type/ L of blood (absolute count). True or False?

A

True

27
Q

When the differential shows an abnormal distribution of cell types, a _ may be performed

A

200-cell differential

28
Q

The results from 200-cell differential are then averaged (divided by 2) and a notation must be made that
200 white cells were counted. True or False?

A

True

29
Q

If the differential count shows the presence of immature granulocytes, this is termed as

A

Shift to the left

30
Q

Refers to an increased number of hyper segmented
neutrophils

A

Shift to the right

31
Q

As the number of total cells counted increases, the amount of variability will _

A

Decreases

32
Q

If NRBCs are seen enumerate them separately and reported as _

A

NRBC/ 100 WBC

33
Q

Reticulocyte is found in
_ & _

A

Bone marrow
Peripheral blood

34
Q

Immature RBC that remains 24 hours in the circulation before they transform into mature RBC

A

Reticulocyte

35
Q

Retics contain remnant of _ and _ which were present in large amount in the cytoplasm

A

Ribosome and RNA

36
Q

An important diagnostic tool which reflects the effectiveness of the bone marrow in producing red blood cells

A

Retics Count

37
Q

The normal value for reticulocyte count

A

0.5-1.5 per 100 RBCs
0.5% to 1.5%
25-75 x 10^9/L

38
Q

Reticulocyte count help in monitoring anemic patients
under treatments; determine the state of increased erythropoietic activity; assess bone marrow activity. True or False?

A

True

39
Q

Corrected Reticulocyte count is calculated based on a normal hematocrit of

A

45%

40
Q

Corrected Reticulocyte Count formula

A

Patient hematocrit/normal hematocrit times Retics count

41
Q

The peripheral blood sample is stained with __

A

Supravital stain

42
Q

Supravital stains are __ that have the ability to react with
_ and _ of reticulocytes while it still alive

A

Basic dyes
Ribosomes and nucleic acids

43
Q

The nucleic acid-dye reaction forms a __ precipitate of granules and filaments

A

Blue

44
Q

Examples of supravital stains

A

Pure azure B
Brilliant cresyl blue
New methylene blue

45
Q

Total number of red cells is equal to

A

Reticulocytes and RBC in 10 fields

46
Q

Retics count normal range in adults

A

0.5% - 1.5%

47
Q

Retics count normal range in neonates

A

2.5% - 6.0%

48
Q

Formula used for Retics counts in Miller Disk

A

A/B(9) x 100

49
Q

___ and use of _ as anti-coagulant can cause
reticulocyte to show pale staining

A

High Blood sugar
Heparin

50
Q

An automated procedure for counting retics using flow
cytometry with fluorescent dyes produces slower yet precise and accurate results. True or False?

A

False

Rationale: Automated procedure for Retics counting produces rapid, precise and accurate results

51
Q

New Methylene Blue can stain inclusions such as

A

Holly-Jowell Bodies
Heinz Bodies
Pappenheimer Bodies
Hemoglobin H

52
Q

These conditions indicates _ retics count?

Effective erythropoiesis
Anemic patient under treatment
Bone marrow assessment after bleeding

A

Increased

53
Q

These conditions indicates _ retics count?

Ineffective erythropoiesis
Hypoproliferative condition

A

Decreased

Hematology 1 (13 decks)

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