Laboratory: Hemoglobin

Question 1

Each hemoglobin molecule consists of the four molecules of globin and four molecules of heme. True or False?

Answer 1

True

Question 2

Hemoglobins may be found in the blood plasma

Answer 2

Hemoglobinemia

Question 3

Hemoglobin is detected
in the urine

Answer 3

Hemoglobinuria

Question 4

Value of hemoglobin may vary according to

Answer 4

Age
Sex
Locality

Question 5

Abnormal increase value in hemoglobin

Answer 5

Hyperchromia

Question 6

Abnormal decrease value in hemoglobin

Answer 6

Hypochromia

Question 7

During the morning, hemoglobin is

Increased

Decreased

Answer 7

Increased

Question 8

High altitude and smokers have _ hemoglobin levels?

Increased

Decreased

Answer 8

Increased

Question 9

During the evening and lying down, hemoglobin is?

Increased

Decreased

Answer 9

Decreased

Question 10

Normal value of hemoglobin in adult male

Answer 10

13-18 g/dL

Question 11

Normal value of hemoglobin in adult female

Answer 11

12-16 g/dL

Question 12

Cyanmethemoglobin: The __ in the reagent converts the hemoglobin iron from the ferrous state to the __

Answer 12

Potassium ferricyanide
Ferric state

Question 13

Cyanmethemoglobin: Methemoglobin which combines with __ to form the stable pigment, ___

Answer 13

Potassium cyanide
Cyanmethemoglobin

Question 14

Cyanmethemoglobin: The __ present in the reagent improves the lysis of the red
blood cells

Answer 14

Non-ionic detergent

Question 15

All forms of hemoglobin are measured with the cyanmetheglobin. True or False?

Answer 15

False

Rationale: Except for sulfhemoglobin

Question 16

Name of reagent used in Cyanmethemoglobin Method

Answer 16

Drabkin’s reagent

Question 17

Cyanmethemoglobin: The dihydrogen potassium phosphate used in the reagent in place of sodium bicarbonate allows the test to be read at the end of 3 minutes. True or False?

Answer 17

True

Question 18

Clouding may be due to an exceptionally high WBC count. What should be done?

Answer 18

Centrifuge the mixture and use the supernatant
as the test sample

Question 19

Clouding may be due to Hemoglobin S and Hemoglobin C. What should be done?

Answer 19

Dilute the mixture 1:1 with distilled water, read on the spectrophotometer, and multiply the result by 2

Question 20

Clouding may be due to lipemic blood. What should be done?

Answer 20

Add 0.01 ml of the patient’s plasma to 5.0 ml of HiCN reagent and use this mixture as the patient blank

Question 21

Over-anticoagulation of the blood does affect the
hemoglobin results. True or False?

Answer 21

False

Rationale: It does not affect the results

Question 22

Acid Hematin: Hemoglobin is converted to acid hematin by _, resulting to __, upon the addition of __

Answer 22

0.1N HC
Brownish yellow color
Distilled water

Question 23

If hemoglobin is converted to an abnormal hemoglobin pigment, it is no longer capable of oxygen transport. True or False?

Answer 23

True

Question 24

Abnormal hemoglobin pigment can cause hypoxia or cyanosis. True or False?

Answer 24

True

Question 25

It is formed by combination of hemoglobin with carbon monoxide

Answer 25

Carboxyhemoglobin

Question 26

The hemoglobin molecule’s affinity for carbon monoxide is more than 500 times greater than for oxygen. True or False?

Answer 26

False Rationale: More than 200 times

Question 27

Carboxyhemoglobin is found in the blood of tobacco smokers in concentration of 170%. True or False?

Answer 27

False Rationale: In concentration of 110%

Question 28

Carboxyhemoglobin can cause headache, nausea, dizziness, and muscular weakness at _ %

Answer 28

20-30%

Question 29

Type of hemoglobin in which the ferrous ion has been oxidized to the ferric state

Answer 29

Methemoglobin

Question 30

Methemoglobinemia may be acquired or inherited. True or False?

Answer 30

True

Question 31

Acquired methemoglobinemia may be a result of structural abnormality of the globin chains, or it may occur as a result of a red blood cell enzyme defect. True or False?

Answer 31

False Rationale: It is Inherited Methemoglobinemia

Question 32

Methemoglobin is normally present in blood at _ %

Answer 32

1-2%

Question 33

Most cases are acquired and are primarily due to exposure to certain drugs. True or False?

Answer 33

True

Question 34

Formed by the action of certain drugs and chemical such as sulfonamides and aromatic amine

Answer 34

Sulfhemoglobin

Question 35

Sulfhemoglobin is incapable of transporting oxygen but can combine with carbon monoxide to form

Answer 35

Carboxysulfhemoglobin

Question 36

Carboxyhemoglobin, Methemglobin and Sulfhemoglobin are all reversible. True or False?

Answer 36

False Rationale: Sulfhemoglobin is irreversible