Laboratory: Hemoglobin Flashcards

1
Q

Each hemoglobin molecule consists of the four molecules of globin and four molecules of heme. True or False?

A

True

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2
Q

Hemoglobins may be found in the blood plasma

A

Hemoglobinemia

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3
Q

Hemoglobin is detected
in the urine

A

Hemoglobinuria

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4
Q

Value of hemoglobin may vary according to

A

Age
Sex
Locality

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5
Q

Abnormal increase value in hemoglobin

A

Hyperchromia

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6
Q

Abnormal decrease value in hemoglobin

A

Hypochromia

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7
Q

During the morning, hemoglobin is

Increased

Decreased

A

Increased

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8
Q

High altitude and smokers have _ hemoglobin levels?

Increased

Decreased

A

Increased

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9
Q

During the evening and lying down, hemoglobin is?

Increased

Decreased

A

Decreased

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10
Q

Normal value of hemoglobin in adult male

A

13-18 g/dL

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11
Q

Normal value of hemoglobin in adult female

A

12-16 g/dL

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12
Q

Cyanmethemoglobin: The __ in the reagent converts the hemoglobin iron from the ferrous state to the __

A

Potassium ferricyanide
Ferric state

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13
Q

Cyanmethemoglobin: Methemoglobin which combines with __ to form the stable pigment, ___

A

Potassium cyanide
Cyanmethemoglobin

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14
Q

Cyanmethemoglobin: The __ present in the reagent improves the lysis of the red
blood cells

A

Non-ionic detergent

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15
Q

All forms of hemoglobin are measured with the cyanmetheglobin. True or False?

A

False

Rationale: Except for sulfhemoglobin

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16
Q

Name of reagent used in Cyanmethemoglobin Method

A

Drabkin’s reagent

17
Q

Cyanmethemoglobin: The dihydrogen potassium phosphate used in the reagent in place of sodium bicarbonate allows the test to be read at the end of 3 minutes. True or False?

A

True

18
Q

Clouding may be due to an exceptionally high WBC count. What should be done?

A

Centrifuge the mixture and use the supernatant
as the test sample

19
Q

Clouding may be due to Hemoglobin S and Hemoglobin C. What should be done?

A

Dilute the mixture 1:1 with distilled water, read on the spectrophotometer, and multiply the result by 2

20
Q

Clouding may be due to lipemic blood. What should be done?

A

Add 0.01 ml of the patient’s plasma to 5.0 ml of HiCN reagent and use this mixture as the patient blank

21
Q

Over-anticoagulation of the blood does affect the
hemoglobin results. True or False?

A

False

Rationale: It does not affect the results

22
Q

Acid Hematin: Hemoglobin is converted to acid hematin by _, resulting to __, upon the addition of __

A

0.1N HC
Brownish yellow color
Distilled water

23
Q

If hemoglobin is converted to an abnormal hemoglobin pigment, it is no longer capable of oxygen transport. True or False?

A

True

24
Q

Abnormal hemoglobin pigment can cause hypoxia or cyanosis. True or False?

A

True

25
Q

It is formed by combination of hemoglobin with carbon monoxide

A

Carboxyhemoglobin

26
Q

The hemoglobin molecule’s affinity for carbon monoxide is more than 500 times greater than for oxygen. True or False?

A

False

Rationale: More than 200 times

27
Q

Carboxyhemoglobin is found in the blood of tobacco smokers in concentration of 170%. True or False?

A

False

Rationale: In concentration of 110%

28
Q

Carboxyhemoglobin can cause headache, nausea, dizziness, and muscular
weakness at _ %

A

20-30%

29
Q

Type of hemoglobin in which the ferrous ion has been oxidized to the ferric state

A

Methemoglobin

30
Q

Methemoglobinemia may be acquired or inherited. True or False?

A

True

31
Q

Acquired methemoglobinemia may be a result of structural
abnormality of the globin chains, or it may occur as a result of a red blood cell enzyme defect. True or False?

A

False

Rationale: It is Inherited Methemoglobinemia

32
Q

Methemoglobin is normally present in blood at _ %

A

1-2%

33
Q

Most cases are acquired and are primarily due to exposure to certain drugs. True or False?

A

True

34
Q

Formed by the action of certain drugs and chemical such as sulfonamides and aromatic amine

A

Sulfhemoglobin

35
Q

Sulfhemoglobin is incapable of transporting oxygen but can combine with carbon monoxide to form

A

Carboxysulfhemoglobin

36
Q

Carboxyhemoglobin, Methemglobin and Sulfhemoglobin are all reversible. True or False?

A

False

Rationale: Sulfhemoglobin is irreversible