Laboratory: Blood Smear Preparation

Question 1

What is the error in preparing the smear if there are holes on monolayer?

Rough edge on spreader slide

Grease on the slide

Uneven pressure on spreader slide

Spreader slide pushed too quickly

Answer 1

Grease on the slide

Question 2

All statements are true about thin smear, except:

This can be done using anticoagulated blood, obtained by finger prick

There are three methods: Slide, cover glass, and spin method

It is used for detection of blood parasites

None of the above

Answer 2

It is used for detection of blood parasites

Question 3

What is the color of the WBC’s nucleus when stained using Wright’s stain?

Answer 3

Purple

Question 4

This is an automated method of preparing thin smear and has the advantage of making red cells free from distortion.

Spin method

Slide method

Cover slip method

Answer 4

Spin method

Question 5

Wright’s, Giemsa, and Leishman are examples of R_________ stain.

Answer 5

Romanowsky

Question 6

What part of the blood smear is examine in obtaining WBC count estimate?

Answer 6

Monolayer

Question 7

In cover slip method, if the drop of blood sits longer than 3-5 seconds, clumping of platelets, WBCs; and coin-stacking formation of RBCs occur. What is the term for “stack of coin” formation of RBC?

Answer 7

Rouleaux

Question 8

How much magnification is needed in performing leukocyte differential count?

Answer 8

100x

Question 9

Which of the following is not a characteristic of a good quality thin smear?

Smooth rounded feather edge

It should cover the entire surface of slide

Thick at one end and progressively thinner

It should be free from waves and holes

Answer 9

It should cover the entire surface of slide

Question 10

What type of smear is well-suitable for the study of morphology of platelets and white cells?

Answer 10

Buffy coat smear

Question 11

What should be done to protect the specimen from physical harm?

Answer 11

Mounting

Question 12

What is the purpose of dehemoglobinization?

to detect parasite in blood smear

to make the smear smooth

to prevent holes and waves during smear preparation

to make the smear faintly pink

Answer 12

To detect parasite in blood smear

Question 13

Special machine used in spin method

Answer 13

Cytospin

Question 14

Oxidation product of methylene blue

Answer 14

Azure B

Question 15

The _ are negatively charged and stain the _ of the cells and _ color

Answer 15

Eosin ions
Basic components
Orange to pink

Question 16

The _ of the cells are stained varying shades of _ by the _

Answer 16

Acid structures
Blue to purple
Positively-charged azure B

Question 17

Wright’s stain is composed of _ and _

Answer 17

Oxidized methylene blue
Eosin

Question 18

Stabilizer used in Giemsa stain

Answer 18

Glycerin

Question 19

Difference between Leishman’s stain and Wright’ stain

Answer 19

Leishman has no oxidized methylene blue

Question 20

Controls the pH of the stain

Answer 20

Phosphate buffer

Question 21

May cause stain precipitate on the dried smear

Answer 21

Insufficient washing of smear

Question 22

In re-staining the slide, _ is used to remove the original stain

Answer 22

Methanol

Question 23

Staining procedures are carried out in _.

Answer 23

Coplin jars

Question 24

Ideal pH of water in staining

Answer 24

6.8

Question 25

Adding triple the quantity of buffered water is recommended in Leishman staining. True or False?

Answer 25

False Rationale: Double the quantity of buffered water

Question 26

Decolorization of smear in Wright staining is done with _ or _ water

Answer 26

Distilled Deionized

Question 27

Temperature required for thick smear preparation

Answer 27

37 degrees Celsius

Question 28

Dehemoglobinization Method 1: Dry the smear, dip in a coplin jar containing _ of _ and _ normal saline

Answer 28

10 drops Glacial acetic acid 50ml

Question 29

Dehemoglobinization Method 2: Without drying immediately put in _ or _ for 15 minutes.

Answer 29

95% ethyl alcohol 5% isopropyl alcohol

Question 30

Stain used for thick smear

Answer 30

Fields Stain A Fields Stain B

Question 31

Examination of Unstained Smear: The rim is then smear with _ to prevent drying up of the blood

Answer 31

Vaseline

Question 32

Fibrinogen, methyl cellulose, and Boyum's reagent are examples of what?

Answer 32

Sedimentation enhancing agents

Question 33

Anticoagulated blood for buffy coat preparation is taken in _ / _ and centrifuged in _

Answer 33

Wintrobe Tube Capillary Tube 300rpm for 15 minute

Question 34

Buffy coat preparation has very much importance because it includes only _ and _

Answer 34

Platelet and WBC

Question 35

In normal case, the thickness of buffy coat layer is _

Answer 35

0.1mm

Question 36

In normal case, plasma is _ color

Answer 36

Straw-yellow

Question 37

Orange color of plasma indicates increased bilirubin. True or False?

Answer 37

True

Question 38

Patients with nephrosis and lipemia have _ or _ plasma layer

Answer 38

Milky Cloudy

Question 39

Quality of the Preparation, Red cell agglutination, Excessive Rouleaux Formation, and Hemoparasites are seen in what magnification?

Answer 39

10x

Question 40

Evaluating WBC Morphology and WBC Count Estimate is done in what magnification?

Answer 40

40x

Question 41

Leukocyte Differential Counts, Leukocyte Morphology and Distribution, Platelet count and morphology is done in what magnification?

Answer 41

100x