Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Hematology 1 > Laboratory: Blood Smear Preparation > Flashcards

Laboratory: Blood Smear Preparation Flashcards

1
Q

What is the error in preparing the smear if there are holes on monolayer?

Rough edge on spreader slide

Grease on the slide

Uneven pressure on spreader slide

Spreader slide pushed too quickly

A

Grease on the slide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

All statements are true about thin smear, except:

This can be done using anticoagulated blood, obtained by finger prick

There are three methods: Slide, cover glass, and spin method

It is used for detection of blood parasites

None of the above

A

It is used for detection of blood parasites

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is the color of the WBC’s nucleus when stained using Wright’s stain?

A

Purple

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

This is an automated method of preparing thin smear and has the advantage of making red cells free from distortion.

Spin method

Slide method

Cover slip method

A

Spin method

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Wright’s, Giemsa, and Leishman are examples of R_________ stain.

A

Romanowsky

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What part of the blood smear is examine in obtaining WBC count estimate?

A

Monolayer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

In cover slip method, if the drop of blood sits longer than 3-5 seconds, clumping of platelets, WBCs; and coin-stacking formation of RBCs occur. What is the term for “stack of coin” formation of RBC?

A

Rouleaux

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

How much magnification is needed in performing leukocyte differential count?

A

100x

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Which of the following is not a characteristic of a good quality thin smear?

Smooth rounded feather edge

It should cover the entire surface of slide

Thick at one end and progressively thinner

It should be free from waves and holes

A

It should cover the entire surface of slide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What type of smear is well-suitable for the study of morphology of platelets and white cells?

A

Buffy coat smear

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What should be done to protect the specimen from physical harm?

A

Mounting

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is the purpose of dehemoglobinization?

to detect parasite in blood smear

to make the smear smooth

to prevent holes and waves during smear preparation

to make the smear faintly pink

A

To detect parasite in blood smear

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Special machine used in spin method

A

Cytospin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Oxidation product of methylene blue

A

Azure B

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

The _ are negatively charged and stain the _ of the cells and _ color

A

Eosin ions
Basic components
Orange to pink

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

The _ of the cells are stained varying shades of _ by the _

A

Acid structures
Blue to purple
Positively-charged azure B

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Wright’s stain is composed of _ and _

A

Oxidized methylene blue
Eosin

18
Q

Stabilizer used in Giemsa stain

A

Glycerin

19
Q

Difference between Leishman’s stain and Wright’ stain

A

Leishman has no oxidized methylene blue

20
Q

Controls the pH of the stain

A

Phosphate buffer

21
Q

May cause stain precipitate on the dried smear

A

Insufficient washing of smear

22
Q

In re-staining the slide, _ is used to remove the original stain

A

Methanol

23
Q

Staining procedures are carried out in _.

A

Coplin jars

24
Q

Ideal pH of water in staining

A

6.8

25
Q

Adding triple the quantity of buffered water is recommended in Leishman staining. True or False?

A

False

Rationale: Double the quantity of buffered water

26
Q

Decolorization of smear in Wright staining is done with _ or _ water

A

Distilled
Deionized

27
Q

Temperature required for thick smear preparation

A

37 degrees Celsius

28
Q

Dehemoglobinization Method 1: Dry the smear, dip in a coplin jar containing _ of _ and _ normal saline

A

10 drops
Glacial acetic acid
50ml

29
Q

Dehemoglobinization Method 2: Without drying immediately put in _ or _ for 15 minutes.

A

95% ethyl alcohol
5% isopropyl alcohol

30
Q

Stain used for thick smear

A

Fields Stain A
Fields Stain B

31
Q

Examination of Unstained Smear: The rim is then smear with _ to prevent drying up of the blood

A

Vaseline

32
Q

Fibrinogen, methyl cellulose, and Boyum’s reagent are examples of what?

A

Sedimentation enhancing agents

33
Q

Anticoagulated blood for buffy coat preparation is taken in _ / _ and centrifuged in _

A

Wintrobe Tube
Capillary Tube
300rpm for 15 minute

34
Q

Buffy coat preparation has very much importance because it includes only _ and _

A

Platelet and WBC

35
Q

In normal case, the thickness of buffy coat layer is _

A

0.1mm

36
Q

In normal case, plasma is _ color

A

Straw-yellow

37
Q

Orange color of plasma indicates increased bilirubin. True or False?

A

True

38
Q

Patients with nephrosis and lipemia have _ or _ plasma layer

A

Milky
Cloudy

39
Q

Quality of the Preparation, Red cell agglutination, Excessive Rouleaux Formation, and Hemoparasites are seen in what magnification?

A

10x

40
Q

Evaluating WBC Morphology and WBC Count Estimate is done in what magnification?

A

40x

41
Q

Leukocyte Differential Counts, Leukocyte Morphology and Distribution, Platelet count and morphology is done in what magnification?

A

100x

Hematology 1 (13 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions