Laboratory: Blood Smear Preparation Flashcards
What is the error in preparing the smear if there are holes on monolayer?
Rough edge on spreader slide
Grease on the slide
Uneven pressure on spreader slide
Spreader slide pushed too quickly
Grease on the slide
All statements are true about thin smear, except:
This can be done using anticoagulated blood, obtained by finger prick
There are three methods: Slide, cover glass, and spin method
It is used for detection of blood parasites
None of the above
It is used for detection of blood parasites
What is the color of the WBC’s nucleus when stained using Wright’s stain?
Purple
This is an automated method of preparing thin smear and has the advantage of making red cells free from distortion.
Spin method
Slide method
Cover slip method
Spin method
Wright’s, Giemsa, and Leishman are examples of R_________ stain.
Romanowsky
What part of the blood smear is examine in obtaining WBC count estimate?
Monolayer
In cover slip method, if the drop of blood sits longer than 3-5 seconds, clumping of platelets, WBCs; and coin-stacking formation of RBCs occur. What is the term for “stack of coin” formation of RBC?
Rouleaux
How much magnification is needed in performing leukocyte differential count?
100x
Which of the following is not a characteristic of a good quality thin smear?
Smooth rounded feather edge
It should cover the entire surface of slide
Thick at one end and progressively thinner
It should be free from waves and holes
It should cover the entire surface of slide
What type of smear is well-suitable for the study of morphology of platelets and white cells?
Buffy coat smear
What should be done to protect the specimen from physical harm?
Mounting
What is the purpose of dehemoglobinization?
to detect parasite in blood smear
to make the smear smooth
to prevent holes and waves during smear preparation
to make the smear faintly pink
To detect parasite in blood smear
Special machine used in spin method
Cytospin
Oxidation product of methylene blue
Azure B
The _ are negatively charged and stain the _ of the cells and _ color
Eosin ions
Basic components
Orange to pink
The _ of the cells are stained varying shades of _ by the _
Acid structures
Blue to purple
Positively-charged azure B
Wright’s stain is composed of _ and _
Oxidized methylene blue
Eosin
Stabilizer used in Giemsa stain
Glycerin
Difference between Leishman’s stain and Wright’ stain
Leishman has no oxidized methylene blue
Controls the pH of the stain
Phosphate buffer
May cause stain precipitate on the dried smear
Insufficient washing of smear
In re-staining the slide, _ is used to remove the original stain
Methanol
Staining procedures are carried out in _.
Coplin jars
Ideal pH of water in staining
6.8
Adding triple the quantity of buffered water is recommended in Leishman staining. True or False?
False
Rationale: Double the quantity of buffered water
Decolorization of smear in Wright staining is done with _ or _ water
Distilled
Deionized
Temperature required for thick smear preparation
37 degrees Celsius
Dehemoglobinization Method 1: Dry the smear, dip in a coplin jar containing _ of _ and _ normal saline
10 drops
Glacial acetic acid
50ml
Dehemoglobinization Method 2: Without drying immediately put in _ or _ for 15 minutes.
95% ethyl alcohol
5% isopropyl alcohol
Stain used for thick smear
Fields Stain A
Fields Stain B
Examination of Unstained Smear: The rim is then smear with _ to prevent drying up of the blood
Vaseline
Fibrinogen, methyl cellulose, and Boyum’s reagent are examples of what?
Sedimentation enhancing agents
Anticoagulated blood for buffy coat preparation is taken in _ / _ and centrifuged in _
Wintrobe Tube
Capillary Tube
300rpm for 15 minute
Buffy coat preparation has very much importance because it includes only _ and _
Platelet and WBC
In normal case, the thickness of buffy coat layer is _
0.1mm
In normal case, plasma is _ color
Straw-yellow
Orange color of plasma indicates increased bilirubin. True or False?
True
Patients with nephrosis and lipemia have _ or _ plasma layer
Milky
Cloudy
Quality of the Preparation, Red cell agglutination, Excessive Rouleaux Formation, and Hemoparasites are seen in what magnification?
10x
Evaluating WBC Morphology and WBC Count Estimate is done in what magnification?
40x
Leukocyte Differential Counts, Leukocyte Morphology and Distribution, Platelet count and morphology is done in what magnification?
100x
